Comparison of translocation of different types of microorganisms from the intestinal tract of burned mice

Tonyia Eaves-Pyles, J. Wesley Alexander

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The aim of this study was to compare the ability of various microorganisms to translocate from the intestine to the mesenteric lymph nodes (MLNs), liver, and spleen in a burned mouse model. Balb/c mice were gavaged with 1 × 109 or 1 × 1010 of one of 11 different microorganisms. All animals were then given a 20% burn. Survival after 10 days showed no significant difference between any of the groups at the 1010 dose. At the 109 dose, significantly higher survival rates were found in three of the 11 strains. Microbial translocation (gavage of 1010 111In-labeled organisms) and host's ability to kill translocated bacteria (viable bacteria in tissues) were measured followed by burn injury and sacrifice four hours later. Translocation and killing of Staphylococcus epidermidis and Escherichia coli was high in the MLNs compared with all other groups but translocation was lower to the liver. Klebsiella, Pseudomonas, and Serratia translocated more evenly to all the tissues. However, these groups showed very high clearance of bacteria in the liver and spleen except for Klebsiella and one strain of Pseudomonas in the spleen. Candida showed poor translocation to all of the tissues and high clearance. It is concluded that various strains of bacteria translocate from the intestine to a similar degree after injury, but the tissues to which they translocate and the rate at which they are killed are somewhat strain dependent.

Original languageEnglish (US)
Pages (from-to)148-152
Number of pages5
JournalShock
Volume16
Issue number2
DOIs
StatePublished - Aug 2001

Keywords

  • Bacterial translocation
  • Gut origin sepsis
  • Intestinal function
  • Systemic infection
  • Thermal injury

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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