Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model

Kumari Lokugamage, Hiroaki Kariwa, Nandadeva Lokugamage, Masahiro Iwasa, Tomohiro Hagiya, Koichi Araki, Atsushi Tachi, Tetsuya Mizutani, Kumiko Yoshimatsu, Jiro Arikawa, Takuya Iwasaki, Ikuo Takashima

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1 focus-forming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50% of the HTN virus inoculated mice survived until 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3×10 6 to 1.3×108 FFU/gram [g] tissue), brain (2.1×107 to 1.2x109 FFU/g tissue), and kidney (2.5×105 to 1.6×107 FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1-5.3×105 FFU/g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice.

Original languageEnglish (US)
Pages (from-to)143-149
Number of pages7
JournalJapanese Journal of Veterinary Research
Volume51
Issue number3-4
StatePublished - Feb 1 2004

Keywords

  • Amur
  • Far east
  • Hantavirus
  • Mouse
  • Virulence

ASJC Scopus subject areas

  • veterinary(all)

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  • Cite this

    Lokugamage, K., Kariwa, H., Lokugamage, N., Iwasa, M., Hagiya, T., Araki, K., Tachi, A., Mizutani, T., Yoshimatsu, K., Arikawa, J., Iwasaki, T., & Takashima, I. (2004). Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model. Japanese Journal of Veterinary Research, 51(3-4), 143-149.