Comparison of wound culture and bronchial lavage in the severely burned child: Implications for antimicrobial therapy

Peter I. Ramzy, David Herndon, Steven Wolf, Oivind Irtun, Juan P. Barret, Roque J. Ramirez, John P. Heggers

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The relationship of the burn wound flora to microbial pathogens in the tracheobronchial tree has important implications for antimicrobial therapy in the severely burned patient. Management of septic complications is bolstered by surveillance quantitative wound cultures (QWC) and bronchial lavage fluid (BLF) cultures. Objectives: To compare the organisms present in BLF with those found in QWC and to determine if QWC can predict BLF results. Design: Results of BLF cultures from all patients who underwent bronchial lavage from January 1, 1996, to December 31, 1996, at our institution were compared with QWC data from the same date. Criteria for a positive match included an identical antibiotic susceptibility pattern and biotype. Match rates were calculated qualitatively and quantitatively. Results: In 30 (48%) of the 62 BLF cultures, there was a match between the organism identified in the BLF and the QWC. When strict quantitative criteria were applied, the match rate was only 9 (14%) of 62. Burn size and inhalation injury had no significant effect on match rate. Conclusions: Whereas the microbial pathogens were similar in the QWC and BLF, linear regression showed no value of QWC in predicting BLF culture results. The difference between qualitative and quantitative match rates suggests cross-colonization between the burn wound and tracheobronchial tree, but little to no cross-infection. The QWC and BLF cultures must be performed independently in determining antimicrobial specificity in the burned patient.

Original languageEnglish (US)
Pages (from-to)1275-1280
Number of pages6
JournalArchives of Surgery
Volume133
Issue number12
DOIs
StatePublished - 1998

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Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid
Wounds and Injuries
Therapeutics
Inhalation Burns
Cross Infection
Linear Models
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Surgery

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Comparison of wound culture and bronchial lavage in the severely burned child : Implications for antimicrobial therapy. / Ramzy, Peter I.; Herndon, David; Wolf, Steven; Irtun, Oivind; Barret, Juan P.; Ramirez, Roque J.; Heggers, John P.

In: Archives of Surgery, Vol. 133, No. 12, 1998, p. 1275-1280.

Research output: Contribution to journalArticle

Ramzy, Peter I. ; Herndon, David ; Wolf, Steven ; Irtun, Oivind ; Barret, Juan P. ; Ramirez, Roque J. ; Heggers, John P. / Comparison of wound culture and bronchial lavage in the severely burned child : Implications for antimicrobial therapy. In: Archives of Surgery. 1998 ; Vol. 133, No. 12. pp. 1275-1280.
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abstract = "Background: The relationship of the burn wound flora to microbial pathogens in the tracheobronchial tree has important implications for antimicrobial therapy in the severely burned patient. Management of septic complications is bolstered by surveillance quantitative wound cultures (QWC) and bronchial lavage fluid (BLF) cultures. Objectives: To compare the organisms present in BLF with those found in QWC and to determine if QWC can predict BLF results. Design: Results of BLF cultures from all patients who underwent bronchial lavage from January 1, 1996, to December 31, 1996, at our institution were compared with QWC data from the same date. Criteria for a positive match included an identical antibiotic susceptibility pattern and biotype. Match rates were calculated qualitatively and quantitatively. Results: In 30 (48{\%}) of the 62 BLF cultures, there was a match between the organism identified in the BLF and the QWC. When strict quantitative criteria were applied, the match rate was only 9 (14{\%}) of 62. Burn size and inhalation injury had no significant effect on match rate. Conclusions: Whereas the microbial pathogens were similar in the QWC and BLF, linear regression showed no value of QWC in predicting BLF culture results. The difference between qualitative and quantitative match rates suggests cross-colonization between the burn wound and tracheobronchial tree, but little to no cross-infection. The QWC and BLF cultures must be performed independently in determining antimicrobial specificity in the burned patient.",
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AU - Heggers, John P.

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