Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model

J. Brian Kimble, Erin Sorrell, Hongxia Shao, Philip L. Martin, Daniel Roberto Perez

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

In 2009, a novel H1N1 influenza (pH1N1) virus caused the first influenza pandemic in 40 y. The virus was identified as a triple reassortant between avian, swine, and human influenza viruses, highlighting the importance of reassortment in the generation of viruses with pandemic potential. Previously, we showed that a reassortant virus composed of wild-type avian H9N2 surface genes in a seasonal human H3N2 backbone could gain efficient respiratory droplet transmission in the ferret model. Here we determine the ability of the H9N2 surface genes in the context of the internal genes of a pH1N1 virus to efficiently transmit via respiratory droplets in ferrets. We generated reassorted viruses carrying the HA gene alone or in combination with the NA gene of a prototypical H9N2 virus in the background of a pH1N1 virus. Four reassortant viruses were generated, with three of them showing efficient respiratory droplet transmission. Differences in replication efficiency were observed for these viruses; however, the results clearly indicate that H9N2 avian influenza viruses and pH1N1 viruses, both of which have occasionally infected pigs, have the potential to reassort and generate novel viruses with respiratory transmission potential in mammals.

Original languageEnglish (US)
Pages (from-to)12084-12088
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number29
DOIs
StatePublished - Jul 19 2011
Externally publishedYes

Fingerprint

Ferrets
Influenza in Birds
Pandemics
Viruses
Genes
Reassortant Viruses
Orthomyxoviridae
Human Influenza
H9N2 Subtype Influenza A Virus
Swine
H1N1 Subtype Influenza A Virus
Mammals

Keywords

  • Ecology
  • Infection
  • Pathology
  • Preparedness
  • Trig cassette

ASJC Scopus subject areas

  • General

Cite this

Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. / Kimble, J. Brian; Sorrell, Erin; Shao, Hongxia; Martin, Philip L.; Perez, Daniel Roberto.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 29, 19.07.2011, p. 12084-12088.

Research output: Contribution to journalArticle

Kimble, J. Brian ; Sorrell, Erin ; Shao, Hongxia ; Martin, Philip L. ; Perez, Daniel Roberto. / Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 29. pp. 12084-12088.
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