Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Jason R. Thonhoff, Dianne I. Lou, Paivi M. Jordan, Xu Zhao, Ping Wu

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

Original languageEnglish (US)
Pages (from-to)42-51
Number of pages10
JournalBrain Research
Volume1187
Issue number1
DOIs
StatePublished - Jan 2 2008

Fingerprint

Fetal Stem Cells
Neural Stem Cells
Hydrogel
Biocompatible Materials
UCON 50-HB-5100
Poloxamer
Hydrogels
Poisons
Stem Cell Transplantation
Tissue Engineering
Spinal Cord Injuries
Brain Injuries
Nervous System
Cell Movement
Regeneration
Cell Differentiation
Stem Cells
Stroke
In Vitro Techniques
Neurons

Keywords

  • Biocompatibility
  • Hydrogel
  • Neural differentiation
  • Scaffold
  • Stem cell
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro. / Thonhoff, Jason R.; Lou, Dianne I.; Jordan, Paivi M.; Zhao, Xu; Wu, Ping.

In: Brain Research, Vol. 1187, No. 1, 02.01.2008, p. 42-51.

Research output: Contribution to journalArticle

Thonhoff, Jason R. ; Lou, Dianne I. ; Jordan, Paivi M. ; Zhao, Xu ; Wu, Ping. / Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro. In: Brain Research. 2008 ; Vol. 1187, No. 1. pp. 42-51.
@article{355240bc98dd4238972e2e9387e16d1f,
title = "Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro",
abstract = "Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30{\%}) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50{\%}) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25{\%}) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.",
keywords = "Biocompatibility, Hydrogel, Neural differentiation, Scaffold, Stem cell, Tissue engineering",
author = "Thonhoff, {Jason R.} and Lou, {Dianne I.} and Jordan, {Paivi M.} and Xu Zhao and Ping Wu",
year = "2008",
month = "1",
day = "2",
doi = "10.1016/j.brainres.2007.10.046",
language = "English (US)",
volume = "1187",
pages = "42--51",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

AU - Thonhoff, Jason R.

AU - Lou, Dianne I.

AU - Jordan, Paivi M.

AU - Zhao, Xu

AU - Wu, Ping

PY - 2008/1/2

Y1 - 2008/1/2

N2 - Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

AB - Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

KW - Biocompatibility

KW - Hydrogel

KW - Neural differentiation

KW - Scaffold

KW - Stem cell

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=36549084987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36549084987&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2007.10.046

DO - 10.1016/j.brainres.2007.10.046

M3 - Article

C2 - 18021754

AN - SCOPUS:36549084987

VL - 1187

SP - 42

EP - 51

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -