Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Jason R. Thonhoff; Dianne I. Lou; Paivi M. Jordan; Xu Zhao; Ping Wu

doi:10.1016/j.brainres.2007.10.046

Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Jason R. Thonhoff, Dianne I. Lou, Paivi M. Jordan, Xu Zhao, Ping Wu

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

Original language	English (US)
Pages (from-to)	42-51
Number of pages	10
Journal	Brain Research
Volume	1187
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2007.10.046
State	Published - Jan 2 2008

Keywords

Biocompatibility
Hydrogel
Neural differentiation
Scaffold
Stem cell
Tissue engineering

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2007.10.046

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title = "Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro",

abstract = "Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.",

keywords = "Biocompatibility, Hydrogel, Neural differentiation, Scaffold, Stem cell, Tissue engineering",

author = "Thonhoff, {Jason R.} and Lou, {Dianne I.} and Jordan, {Paivi M.} and Xu Zhao and Ping Wu",

note = "Funding Information: The authors thank the contributions from Drs. Joan Nichols and Joaquin Cortiella for critical scientific discussion and for providing PGA and PF127. The authors also thank the technical assistance from Tiffany J. Dunn and the critical review by Dr. Richard Coggeshall. Sponsorship: Supported by the National Institute of Neurological Disorders and Stroke (NS046025), the Coalition for Brain Injury Research, the TIRR Foundation and the John S. Dunn Research Foundation.",

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AU - Thonhoff, Jason R.

AU - Lou, Dianne I.

AU - Jordan, Paivi M.

AU - Zhao, Xu

AU - Wu, Ping

N1 - Funding Information: The authors thank the contributions from Drs. Joan Nichols and Joaquin Cortiella for critical scientific discussion and for providing PGA and PF127. The authors also thank the technical assistance from Tiffany J. Dunn and the critical review by Dr. Richard Coggeshall. Sponsorship: Supported by the National Institute of Neurological Disorders and Stroke (NS046025), the Coalition for Brain Injury Research, the TIRR Foundation and the John S. Dunn Research Foundation.

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N2 - Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

AB - Stroke and spinal cord or brain injury often result in cavity formation. Stem cell transplantation in combination with tissue engineering has the potential to fill such a cavity and replace lost neurons. Several hydrogels containing unique features particularly suitable for the delicate nervous system were tested by determining whether these materials were compatible with fetal human neural stem cells (hNSCs) in terms of toxicity and ability to support stem cell differentiation in vitro. The hydrogels examined were pluronic F127 (PF127), Matrigel and PuraMatrix. We found that PF127, in a gelated (30%) form, was toxic to hNSCs, and Matrigel, in a gelated (1-50%) form, prevented hNSCs' normal capacity for neuronal differentiation. In contrast, PuraMatrix was the most optimal hydrogel for hNSCs, since it showed low toxicity when gelated (0.25%) and retained several crucial properties of hNSCs, including migration and neuronal differentiation. Further optimization and characterization of PuraMatrix is warranted to explore its full potential in assisting neural regeneration in vivo.

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Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro

Abstract

Keywords

ASJC Scopus subject areas

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