Complement C3 production in human intestinal epithelial cells is regulated by interleukin 1β and tumor necrosis factor α

Ryan Moon, Alexander A. Parikh, Csaba Szabo, Josef E. Fischer, Andrew L. Salzman, Per Olof Hasselgren

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Sepsis and endotoxemia are associated with increased mucosal production of complement component C3; the enterocyte may be a source of C3 in these conditions. Objective: To test the hypothesis that interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) regulate the production of C3 in the enterocyte at the transcriptional level and that this regulation is potentiated by interferon gamma (IFN-γ). Methods: Cultured Caco-2 cells, a human intestinal epithelial cell line, were treated with various concentrations of human recombinant IL-1β (0.005-1.25 ng/mL) or TNF-α (1- 1000 U/mL) with or without the addition of IFN-γ (250 U/mL). C3 levels in the culture medium were measured by enzyme-linked immunosorbent assay and cellular messenger RNA levels by Northern blot analysis. Results: Treatment of the Caco-2 cells with IL-1β or TNF-α resulted in a time- and dose- dependent increase in C3 production. The use of IFN-γ alone did not affect C3 production but potentiated the effect of IL-1β and TNF-α in a synergistic manner. C3 messenger RNA levels were increased following stimulation with either cytokine. Conclusions: C3 production in the enterocyte is regulated by IL-1β and TNF-α at the transcriptional level, and this response is potentiated by IFN-γ. The results suggest that C3 production in the intestinal mucosa may be regulated locally by cytokines in a paracrine or autocrine manner.

Original languageEnglish (US)
Pages (from-to)1289-1293
Number of pages5
JournalArchives of Surgery
Volume132
Issue number12
StatePublished - Dec 1997
Externally publishedYes

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Complement C3
Interleukin-1
Tumor Necrosis Factor-alpha
Epithelial Cells
Interferon-gamma
Enterocytes
Caco-2 Cells
Cytokines
Messenger RNA
Endotoxemia
Intestinal Mucosa
Northern Blotting
Culture Media
Cultured Cells
Sepsis
Enzyme-Linked Immunosorbent Assay
Cell Line

ASJC Scopus subject areas

  • Surgery

Cite this

Moon, R., Parikh, A. A., Szabo, C., Fischer, J. E., Salzman, A. L., & Hasselgren, P. O. (1997). Complement C3 production in human intestinal epithelial cells is regulated by interleukin 1β and tumor necrosis factor α. Archives of Surgery, 132(12), 1289-1293.

Complement C3 production in human intestinal epithelial cells is regulated by interleukin 1β and tumor necrosis factor α. / Moon, Ryan; Parikh, Alexander A.; Szabo, Csaba; Fischer, Josef E.; Salzman, Andrew L.; Hasselgren, Per Olof.

In: Archives of Surgery, Vol. 132, No. 12, 12.1997, p. 1289-1293.

Research output: Contribution to journalArticle

Moon, R, Parikh, AA, Szabo, C, Fischer, JE, Salzman, AL & Hasselgren, PO 1997, 'Complement C3 production in human intestinal epithelial cells is regulated by interleukin 1β and tumor necrosis factor α', Archives of Surgery, vol. 132, no. 12, pp. 1289-1293.
Moon, Ryan ; Parikh, Alexander A. ; Szabo, Csaba ; Fischer, Josef E. ; Salzman, Andrew L. ; Hasselgren, Per Olof. / Complement C3 production in human intestinal epithelial cells is regulated by interleukin 1β and tumor necrosis factor α. In: Archives of Surgery. 1997 ; Vol. 132, No. 12. pp. 1289-1293.
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