Complement depletion affects demyelination and inflammation in experimental allergic neuritis

Francine J. Vriesendorp, Robyn E. Flynn, Miguel Pappolla, Carol L. Koski

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (BAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 ± 0.7 vs. 1.1 ± 1.1), less demyelination (0.4 ± 0.8 vs. 1.9 ± 1.1) and inflammation (0.6 ± 1.2 vs. 2 ± 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer EDI-positive (phagocytic) macrophages (if0.4 ± 0.5 vs.> 1.6 ± 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 ± 0.8 vs. 2.5 ± 0.8) (mean ± S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Neuroimmunology
Volume58
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Experimental Autoimmune Neuritis
Demyelinating Diseases
Myelin Sheath
Inflammation
Peripheral Nerves
Macrophages
Macrophage-1 Antigen
Complement C3b
Phagocytosis
Immunohistochemistry

Keywords

  • Complement
  • Demyelination
  • Experimental allergic neuritis
  • Macrophage

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Complement depletion affects demyelination and inflammation in experimental allergic neuritis. / Vriesendorp, Francine J.; Flynn, Robyn E.; Pappolla, Miguel; Koski, Carol L.

In: Journal of Neuroimmunology, Vol. 58, No. 2, 1995, p. 157-165.

Research output: Contribution to journalArticle

Vriesendorp, Francine J. ; Flynn, Robyn E. ; Pappolla, Miguel ; Koski, Carol L. / Complement depletion affects demyelination and inflammation in experimental allergic neuritis. In: Journal of Neuroimmunology. 1995 ; Vol. 58, No. 2. pp. 157-165.
@article{642f2465e1284f0ca783b53dd6f10c7c,
title = "Complement depletion affects demyelination and inflammation in experimental allergic neuritis",
abstract = "The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (BAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 ± 0.7 vs. 1.1 ± 1.1), less demyelination (0.4 ± 0.8 vs. 1.9 ± 1.1) and inflammation (0.6 ± 1.2 vs. 2 ± 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer EDI-positive (phagocytic) macrophages (if0.4 ± 0.5 vs.> 1.6 ± 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 ± 0.8 vs. 2.5 ± 0.8) (mean ± S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis.",
keywords = "Complement, Demyelination, Experimental allergic neuritis, Macrophage",
author = "Vriesendorp, {Francine J.} and Flynn, {Robyn E.} and Miguel Pappolla and Koski, {Carol L.}",
year = "1995",
doi = "10.1016/0165-5728(95)00006-N",
language = "English (US)",
volume = "58",
pages = "157--165",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Complement depletion affects demyelination and inflammation in experimental allergic neuritis

AU - Vriesendorp, Francine J.

AU - Flynn, Robyn E.

AU - Pappolla, Miguel

AU - Koski, Carol L.

PY - 1995

Y1 - 1995

N2 - The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (BAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 ± 0.7 vs. 1.1 ± 1.1), less demyelination (0.4 ± 0.8 vs. 1.9 ± 1.1) and inflammation (0.6 ± 1.2 vs. 2 ± 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer EDI-positive (phagocytic) macrophages (if0.4 ± 0.5 vs.> 1.6 ± 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 ± 0.8 vs. 2.5 ± 0.8) (mean ± S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis.

AB - The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (BAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 ± 0.7 vs. 1.1 ± 1.1), less demyelination (0.4 ± 0.8 vs. 1.9 ± 1.1) and inflammation (0.6 ± 1.2 vs. 2 ± 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer EDI-positive (phagocytic) macrophages (if0.4 ± 0.5 vs.> 1.6 ± 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 ± 0.8 vs. 2.5 ± 0.8) (mean ± S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis.

KW - Complement

KW - Demyelination

KW - Experimental allergic neuritis

KW - Macrophage

UR - http://www.scopus.com/inward/record.url?scp=0029044838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029044838&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(95)00006-N

DO - 10.1016/0165-5728(95)00006-N

M3 - Article

C2 - 7759605

AN - SCOPUS:0029044838

VL - 58

SP - 157

EP - 165

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 2

ER -