TY - JOUR
T1 - Complement-mediated release of histamine from human basophils. III. Possible regulatory role of microtubules and microfilaments
AU - Grant, J. Andrew
AU - Dupree, Elton
AU - Thueson, David O.
PY - 1977/11
Y1 - 1977/11
N2 - The release of histamine by normal human leukocytes (basophils) following in vitro challenge with activated complement (zymosan-treated serum) was previously reported. In this study, the effects of various pharmacologic agents on this release mechanism were compared with allergen-induced release of histamine. Colchicine and vinblastine antagonize the polymerization of tubulin to form microtubules, and both agents inhibited complement- and allergen-mediated release of histamine. The dose-response effects of these drugs were quite similar for the two release mechanisms. Deuterium oxide, an agent which stabilizes microtubules, enhanced both complement- and allergen-triggered release of histamine from basophils. Finally, treatment with cytochalasin B, a fungal product known to interfere with microfilament formation, resulted in enhanced release of histamine from complement-treated basophils but no significant change in the percentage of histamine released from allergen-treated basophils. These findings suggest that microtubules and/or microfilaments are involved in complement-induced secretion of histamine by human basophils.
AB - The release of histamine by normal human leukocytes (basophils) following in vitro challenge with activated complement (zymosan-treated serum) was previously reported. In this study, the effects of various pharmacologic agents on this release mechanism were compared with allergen-induced release of histamine. Colchicine and vinblastine antagonize the polymerization of tubulin to form microtubules, and both agents inhibited complement- and allergen-mediated release of histamine. The dose-response effects of these drugs were quite similar for the two release mechanisms. Deuterium oxide, an agent which stabilizes microtubules, enhanced both complement- and allergen-triggered release of histamine from basophils. Finally, treatment with cytochalasin B, a fungal product known to interfere with microfilament formation, resulted in enhanced release of histamine from complement-treated basophils but no significant change in the percentage of histamine released from allergen-treated basophils. These findings suggest that microtubules and/or microfilaments are involved in complement-induced secretion of histamine by human basophils.
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U2 - 10.1016/0091-6749(77)90110-5
DO - 10.1016/0091-6749(77)90110-5
M3 - Article
C2 - 72086
AN - SCOPUS:0017705521
SN - 0091-6749
VL - 60
SP - 306
EP - 311
JO - The Journal of Allergy and Clinical Immunology
JF - The Journal of Allergy and Clinical Immunology
IS - 5
ER -