Complement mediated release of histamine from human leukocytes

J. A. Grant, E. Dupree, A. Goldman, D. R. Schultz, A. L. Jackson

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Activation of either the alternative or classical pathway of complement generated a factor which induced release of histamine from both non allergic and allergic human basophils. This factor probably is derived from the complement system since its formation was associated with loss of C3 activity in human serum, chemotactic factor (probably also a complement product) was generated simultaneously, heat inactivation blocked its formation, anti C3 and anti C5 blocked formation of the factor, and finally anti C5 inhibited the activity of the factor once it had been formed. It appears that both complement mediated and allergen mediated release of histamine from basophils are secretory, noncytolytic pathways since both were maximal at 37°C, required the presence of divalent cations, and were inhibited by theophylline. One consistent difference between these two mechanisms was noted: complement initiated release of histamine occurred more quickly.

Original languageEnglish (US)
Pages (from-to)1101-1106
Number of pages6
JournalJournal of Immunology
Volume114
Issue number3
StatePublished - 1975

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Histamine Release
Leukocytes
Basophils
Classical Complement Pathway
Secretory Pathway
Chemotactic Factors
Divalent Cations
Theophylline
Human Activities
Allergens
Hot Temperature
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Grant, J. A., Dupree, E., Goldman, A., Schultz, D. R., & Jackson, A. L. (1975). Complement mediated release of histamine from human leukocytes. Journal of Immunology, 114(3), 1101-1106.

Complement mediated release of histamine from human leukocytes. / Grant, J. A.; Dupree, E.; Goldman, A.; Schultz, D. R.; Jackson, A. L.

In: Journal of Immunology, Vol. 114, No. 3, 1975, p. 1101-1106.

Research output: Contribution to journalArticle

Grant, JA, Dupree, E, Goldman, A, Schultz, DR & Jackson, AL 1975, 'Complement mediated release of histamine from human leukocytes', Journal of Immunology, vol. 114, no. 3, pp. 1101-1106.
Grant JA, Dupree E, Goldman A, Schultz DR, Jackson AL. Complement mediated release of histamine from human leukocytes. Journal of Immunology. 1975;114(3):1101-1106.
Grant, J. A. ; Dupree, E. ; Goldman, A. ; Schultz, D. R. ; Jackson, A. L. / Complement mediated release of histamine from human leukocytes. In: Journal of Immunology. 1975 ; Vol. 114, No. 3. pp. 1101-1106.
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