Complement protective epitopes and CD55-microtubule complexes facilitate the invasion and intracellular persistence of uropathogenic escherichia coli

Tanu Rana, Rafia J. Hasan, Stella Nowicki, Mathura S. Venkatarajan, Rajbir Singh, Petri T. Urvil, Vsevolod Popov, Werner Braun, Waldemar Popik, J. Shawn Goodwin, Bogdan J. Nowicki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. Escherichia coli-bearing Dr-adhesins (Dr+ E. coli) cause chronic pyelonephritis in pregnant women and animal models. This chronic renal infection correlates with the capacity of bacteria to invade epithelial cells expressing CD55. The mechanism of infection remains unknown.Methods. CD55 amino acids in the vicinity of binding pocket-Ser155 for Dr-adhesin were mutated to alanine and subjected to temporal gentamicin-invasion/gentamicin-survival assay in Chinese hamster ovary cells. CD55/microtubule (MT) responses were studied using confocal/electron microscopy, and 3-dimensional structure analysis.Results. Mutant analysis revealed that complement-protective CD55-Ser165 and CD55-Phe154 epitopes control E. coli invasion by coregulating CD55-MT complex expression. Single-point CD55 mutations changed E. coli to either a minimally invasive (Ser165Ala) or a hypervirulent pathogen (Phe154Ala). Thus, single amino acid modifications with no impact on CD55 structure and bacterial attachment can have a profound impact on E. coli virulence. While CD55-Ser165Ala decreased E. coli invasion and led to dormant intracellular persistence, intracellular E. coli in CD55-Phe154Ala developed elongated forms (multiplying within vacuoles), upregulated CD55-MT complexes, acquired CD55 coat, and escaped phagolysosomal fusion.Conclusions. E. coli target complement-protective CD55 epitopes for invasion and exploit CD55-MT complexes to escape phagolysosomal fusion, leading to a nondestructive parasitism that allows bacteria to persist intracellularly.

Original languageEnglish
Pages (from-to)1066-1076
Number of pages11
JournalJournal of Infectious Diseases
Volume209
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Uropathogenic Escherichia coli
Microtubules
Epitopes
Escherichia coli
Gentamicins
Bacteria
Bacterial Structures
Amino Acids
Pyelonephritis
Vacuoles
Cricetulus
Infection
Point Mutation
Confocal Microscopy
Alanine
Virulence
Pregnant Women
Ovary
Electron Microscopy
Animal Models

Keywords

  • CD55
  • intracellular persistence
  • invasion
  • microtubules

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Complement protective epitopes and CD55-microtubule complexes facilitate the invasion and intracellular persistence of uropathogenic escherichia coli. / Rana, Tanu; Hasan, Rafia J.; Nowicki, Stella; Venkatarajan, Mathura S.; Singh, Rajbir; Urvil, Petri T.; Popov, Vsevolod; Braun, Werner; Popik, Waldemar; Goodwin, J. Shawn; Nowicki, Bogdan J.

In: Journal of Infectious Diseases, Vol. 209, No. 7, 2014, p. 1066-1076.

Research output: Contribution to journalArticle

Rana, Tanu ; Hasan, Rafia J. ; Nowicki, Stella ; Venkatarajan, Mathura S. ; Singh, Rajbir ; Urvil, Petri T. ; Popov, Vsevolod ; Braun, Werner ; Popik, Waldemar ; Goodwin, J. Shawn ; Nowicki, Bogdan J. / Complement protective epitopes and CD55-microtubule complexes facilitate the invasion and intracellular persistence of uropathogenic escherichia coli. In: Journal of Infectious Diseases. 2014 ; Vol. 209, No. 7. pp. 1066-1076.
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abstract = "Background. Escherichia coli-bearing Dr-adhesins (Dr+ E. coli) cause chronic pyelonephritis in pregnant women and animal models. This chronic renal infection correlates with the capacity of bacteria to invade epithelial cells expressing CD55. The mechanism of infection remains unknown.Methods. CD55 amino acids in the vicinity of binding pocket-Ser155 for Dr-adhesin were mutated to alanine and subjected to temporal gentamicin-invasion/gentamicin-survival assay in Chinese hamster ovary cells. CD55/microtubule (MT) responses were studied using confocal/electron microscopy, and 3-dimensional structure analysis.Results. Mutant analysis revealed that complement-protective CD55-Ser165 and CD55-Phe154 epitopes control E. coli invasion by coregulating CD55-MT complex expression. Single-point CD55 mutations changed E. coli to either a minimally invasive (Ser165Ala) or a hypervirulent pathogen (Phe154Ala). Thus, single amino acid modifications with no impact on CD55 structure and bacterial attachment can have a profound impact on E. coli virulence. While CD55-Ser165Ala decreased E. coli invasion and led to dormant intracellular persistence, intracellular E. coli in CD55-Phe154Ala developed elongated forms (multiplying within vacuoles), upregulated CD55-MT complexes, acquired CD55 coat, and escaped phagolysosomal fusion.Conclusions. E. coli target complement-protective CD55 epitopes for invasion and exploit CD55-MT complexes to escape phagolysosomal fusion, leading to a nondestructive parasitism that allows bacteria to persist intracellularly.",
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AU - Rana, Tanu

AU - Hasan, Rafia J.

AU - Nowicki, Stella

AU - Venkatarajan, Mathura S.

AU - Singh, Rajbir

AU - Urvil, Petri T.

AU - Popov, Vsevolod

AU - Braun, Werner

AU - Popik, Waldemar

AU - Goodwin, J. Shawn

AU - Nowicki, Bogdan J.

PY - 2014

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N2 - Background. Escherichia coli-bearing Dr-adhesins (Dr+ E. coli) cause chronic pyelonephritis in pregnant women and animal models. This chronic renal infection correlates with the capacity of bacteria to invade epithelial cells expressing CD55. The mechanism of infection remains unknown.Methods. CD55 amino acids in the vicinity of binding pocket-Ser155 for Dr-adhesin were mutated to alanine and subjected to temporal gentamicin-invasion/gentamicin-survival assay in Chinese hamster ovary cells. CD55/microtubule (MT) responses were studied using confocal/electron microscopy, and 3-dimensional structure analysis.Results. Mutant analysis revealed that complement-protective CD55-Ser165 and CD55-Phe154 epitopes control E. coli invasion by coregulating CD55-MT complex expression. Single-point CD55 mutations changed E. coli to either a minimally invasive (Ser165Ala) or a hypervirulent pathogen (Phe154Ala). Thus, single amino acid modifications with no impact on CD55 structure and bacterial attachment can have a profound impact on E. coli virulence. While CD55-Ser165Ala decreased E. coli invasion and led to dormant intracellular persistence, intracellular E. coli in CD55-Phe154Ala developed elongated forms (multiplying within vacuoles), upregulated CD55-MT complexes, acquired CD55 coat, and escaped phagolysosomal fusion.Conclusions. E. coli target complement-protective CD55 epitopes for invasion and exploit CD55-MT complexes to escape phagolysosomal fusion, leading to a nondestructive parasitism that allows bacteria to persist intracellularly.

AB - Background. Escherichia coli-bearing Dr-adhesins (Dr+ E. coli) cause chronic pyelonephritis in pregnant women and animal models. This chronic renal infection correlates with the capacity of bacteria to invade epithelial cells expressing CD55. The mechanism of infection remains unknown.Methods. CD55 amino acids in the vicinity of binding pocket-Ser155 for Dr-adhesin were mutated to alanine and subjected to temporal gentamicin-invasion/gentamicin-survival assay in Chinese hamster ovary cells. CD55/microtubule (MT) responses were studied using confocal/electron microscopy, and 3-dimensional structure analysis.Results. Mutant analysis revealed that complement-protective CD55-Ser165 and CD55-Phe154 epitopes control E. coli invasion by coregulating CD55-MT complex expression. Single-point CD55 mutations changed E. coli to either a minimally invasive (Ser165Ala) or a hypervirulent pathogen (Phe154Ala). Thus, single amino acid modifications with no impact on CD55 structure and bacterial attachment can have a profound impact on E. coli virulence. While CD55-Ser165Ala decreased E. coli invasion and led to dormant intracellular persistence, intracellular E. coli in CD55-Phe154Ala developed elongated forms (multiplying within vacuoles), upregulated CD55-MT complexes, acquired CD55 coat, and escaped phagolysosomal fusion.Conclusions. E. coli target complement-protective CD55 epitopes for invasion and exploit CD55-MT complexes to escape phagolysosomal fusion, leading to a nondestructive parasitism that allows bacteria to persist intracellularly.

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