Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae

Michael P. McLeod, Xiang Qin, Sandor E. Karpathy, Jason Gioia, Sarah K. Highlander, George E. Fox, Thomas Z. McNeill, Huaiyang Jiang, Donna Muzny, Leni S. Jacob, Alicia C. Hawes, Erica Sodergren, Rachel Gill, Jennifer Hume, Maggie Morgan, Guangwei Fan, Anita G. Amin, Richard A. Gibbs, Chao Hong, Xue Jie Yu & 2 others David Walker, George M. Weinstock

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and R. conorii. We identified 877 genes in R. typhi encoding 3 rRNAs, 33 tRNAs, 3 noncoding RNAs, and 838 proteins, 3 of which are frameshifts. In addition, we discovered more than 40 pseudogenes, including the entire cytochrome c oxidase system. The three rickettsial genomes share 775 genes: 23 are found only in R. prowazekii and R. typhi, 15 are found only in R. conorii and R. typhi, and 24 are unique to R. typhi. Although most of the genes are colinear, there is a 35-kb inversion in gene order, which is close to the replication terminus, in R. typhi, compared to R. prowazekii and R. conorii. In addition, we found a 124-kb R. typhi-specific inversion, starting 19 kb from the origin of replication, compared to R. prowazekii and R. conorii. Inversions in this region are also seen in the unpublished genome sequences of R. sibirica and R. rickettsii, indicating that this region is a hot spot for rearrangements. Genome comparisons also revealed a 12-kb insertion in the R. prowazekii genome, relative to R. typhi and R. conorii, which appears to have occurred after the typhus (R. prowazekii and R typhi) and spotted fever (R. conorii) groups diverged. The three-way comparison allowed further in silico analysis of the SpoT split genes, leading us to propose that the stringent response system is still functional in these rickettsiae.

Original languageEnglish (US)
Pages (from-to)5842-5855
Number of pages14
JournalJournal of Bacteriology
Volume186
Issue number17
DOIs
StatePublished - Sep 2004

Fingerprint

Rickettsia typhi
Rickettsia
Genome
Genes
Endemic Flea-Borne Typhus
Epidemic Louse-Borne Typhus
Untranslated RNA
Pseudogenes
Replication Origin
Gene Order
Electron Transport Complex IV
Invertebrates
Transfer RNA
Life Cycle Stages
Computer Simulation
Vertebrates
Fever

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

McLeod, M. P., Qin, X., Karpathy, S. E., Gioia, J., Highlander, S. K., Fox, G. E., ... Weinstock, G. M. (2004). Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae. Journal of Bacteriology, 186(17), 5842-5855. https://doi.org/10.1128/JB.186.17.5842-5855.2004

Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae. / McLeod, Michael P.; Qin, Xiang; Karpathy, Sandor E.; Gioia, Jason; Highlander, Sarah K.; Fox, George E.; McNeill, Thomas Z.; Jiang, Huaiyang; Muzny, Donna; Jacob, Leni S.; Hawes, Alicia C.; Sodergren, Erica; Gill, Rachel; Hume, Jennifer; Morgan, Maggie; Fan, Guangwei; Amin, Anita G.; Gibbs, Richard A.; Hong, Chao; Yu, Xue Jie; Walker, David; Weinstock, George M.

In: Journal of Bacteriology, Vol. 186, No. 17, 09.2004, p. 5842-5855.

Research output: Contribution to journalArticle

McLeod, MP, Qin, X, Karpathy, SE, Gioia, J, Highlander, SK, Fox, GE, McNeill, TZ, Jiang, H, Muzny, D, Jacob, LS, Hawes, AC, Sodergren, E, Gill, R, Hume, J, Morgan, M, Fan, G, Amin, AG, Gibbs, RA, Hong, C, Yu, XJ, Walker, D & Weinstock, GM 2004, 'Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae', Journal of Bacteriology, vol. 186, no. 17, pp. 5842-5855. https://doi.org/10.1128/JB.186.17.5842-5855.2004
McLeod, Michael P. ; Qin, Xiang ; Karpathy, Sandor E. ; Gioia, Jason ; Highlander, Sarah K. ; Fox, George E. ; McNeill, Thomas Z. ; Jiang, Huaiyang ; Muzny, Donna ; Jacob, Leni S. ; Hawes, Alicia C. ; Sodergren, Erica ; Gill, Rachel ; Hume, Jennifer ; Morgan, Maggie ; Fan, Guangwei ; Amin, Anita G. ; Gibbs, Richard A. ; Hong, Chao ; Yu, Xue Jie ; Walker, David ; Weinstock, George M. / Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 17. pp. 5842-5855.
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AU - Gill, Rachel

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AU - Amin, Anita G.

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