Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes

Donald J. Netolitzky, Fay L. Schmaltz, Michael D. Parker, George A. Rayner, Glen R. Fisher, Dennis W. Trent, Douglas E. Bader, Les P. Nagata

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The complete nucleotide sequence of the 71V-1658 strain of western equine encephalitis virus (WEE) was determined (minus 25 nucleotides from the 5' end). A 5' RACE reaction was used to sequence the 5' terminus from WEE strain CBA87. The deduced WEE genome was 11508 nucleotides in length, excluding the 5' cap nucleotide and 3' poly(A) tail. The nucleotide composition was 28% A, 25% C, 25% G and 22% U. Comparison with partial WEE sequences of strain 5614 (nsP2-nsP3 of the nonstructural region) and strain BFS 1703 (26S structural region) revealed comparatively little variation; a total of 149 nucleotide differences in 8624 bases (1.7% divergence), of which only 28% (42 nucleotides) altered the encoded amino acids. Comparison of deduced nsP1 and nsP4 amino acid sequences from WEE with the corresponding proteins from eastern equine encephalitis virus (EEE) yielded identities of 84.9 and 83.8%, respectively. Previously uncharacterized stem-loop structures were identified in the nontranslated terminal regions. A cDNA clone of the 26S region encoding the structural polyprotein of WEE strain 71V-1658 was placed under the control of a cytomegalovirus promoter and transfected into tissue culture cells, The viral envelope proteins were functionally expressed in tissue culture as determined by histochemical staining with monoclonal antibodies that recognize WEE antigens, thus, forming the initial step in the investigation of subunit vaccines to WEE.

Original languageEnglish (US)
Pages (from-to)151-159
Number of pages9
JournalJournal of General Virology
Volume81
Issue number1
StatePublished - 2000

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Western Equine Encephalitis Viruses
Gene Expression
Nucleotides
Eastern equine encephalitis virus
Viral Envelope Proteins
Polyproteins
Subunit Vaccines
Cytomegalovirus
Amino Acid Sequence
Complementary DNA
Clone Cells
Cell Culture Techniques
Monoclonal Antibodies
Genome
Staining and Labeling
Antigens
Amino Acids

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Netolitzky, D. J., Schmaltz, F. L., Parker, M. D., Rayner, G. A., Fisher, G. R., Trent, D. W., ... Nagata, L. P. (2000). Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes. Journal of General Virology, 81(1), 151-159.

Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes. / Netolitzky, Donald J.; Schmaltz, Fay L.; Parker, Michael D.; Rayner, George A.; Fisher, Glen R.; Trent, Dennis W.; Bader, Douglas E.; Nagata, Les P.

In: Journal of General Virology, Vol. 81, No. 1, 2000, p. 151-159.

Research output: Contribution to journalArticle

Netolitzky, DJ, Schmaltz, FL, Parker, MD, Rayner, GA, Fisher, GR, Trent, DW, Bader, DE & Nagata, LP 2000, 'Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes', Journal of General Virology, vol. 81, no. 1, pp. 151-159.
Netolitzky DJ, Schmaltz FL, Parker MD, Rayner GA, Fisher GR, Trent DW et al. Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes. Journal of General Virology. 2000;81(1):151-159.
Netolitzky, Donald J. ; Schmaltz, Fay L. ; Parker, Michael D. ; Rayner, George A. ; Fisher, Glen R. ; Trent, Dennis W. ; Bader, Douglas E. ; Nagata, Les P. / Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes. In: Journal of General Virology. 2000 ; Vol. 81, No. 1. pp. 151-159.
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