Complete hydatidiform mole retaining a chromosome 11 of maternal origin

Molecular genetic analysis of a case

Rosemary A. Fisher, Marisa R. Nucci, Harshwardhan Thaker, Stanislawa Weremowicz, David R. Genest, Diego H. Castrillon

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Hydatidiform moles are pregnancies characterized by abnormal development of both embryonic and extraembryonic tissues and are associated with the misexpression of imprinted genes. The vast majority of complete hydatidiform moles are diploid and androgenetic, whereas partial hydatidiform moles are triploid, with an extra set of chromosomes of paternal origin. Here, we present an unusual complete mole that showed strong expression of two imprinted, maternally transcribed genes, CDKN1C (encoding p57KIP2) and PHLDA2 (TSSC3/IPL), both part of a large imprinted gene domain on chromosome 11. Using microsatellite genotyping and fluorescent in situ hybridization, we show that this paradoxical gene expression was due to retention of a maternal copy of chromosome 11 in addition to the two paternal copies normally present in complete moles. These findings demonstrate that, despite being predominantly androgenetic, some complete moles contain small amounts of DNA of maternal origin. Furthermore, these results provide an explanation for rare false negatives that can arise when p57KIP2 is used as a diagnostic marker for complete moles.

Original languageEnglish (US)
Pages (from-to)1155-1160
Number of pages6
JournalModern Pathology
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Fingerprint

Hydatidiform Mole
Chromosomes, Human, Pair 11
Molecular Biology
Mothers
Genes
Triploidy
Diploidy
Fluorescence In Situ Hybridization
Microsatellite Repeats
Embryonic Development
Chromosomes
Gene Expression
Pregnancy
DNA

Keywords

  • CDKN1C
  • Genomic imprinting
  • Hydatidiform mole
  • IPL
  • P57
  • PHLDA2
  • Trisomy 11

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Complete hydatidiform mole retaining a chromosome 11 of maternal origin : Molecular genetic analysis of a case. / Fisher, Rosemary A.; Nucci, Marisa R.; Thaker, Harshwardhan; Weremowicz, Stanislawa; Genest, David R.; Castrillon, Diego H.

In: Modern Pathology, Vol. 17, No. 9, 01.09.2004, p. 1155-1160.

Research output: Contribution to journalArticle

Fisher, Rosemary A. ; Nucci, Marisa R. ; Thaker, Harshwardhan ; Weremowicz, Stanislawa ; Genest, David R. ; Castrillon, Diego H. / Complete hydatidiform mole retaining a chromosome 11 of maternal origin : Molecular genetic analysis of a case. In: Modern Pathology. 2004 ; Vol. 17, No. 9. pp. 1155-1160.
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