Complex adenovirus-mediated expression of West Nile Virus C, preM, E, and NS1 proteins induces both humoral and cellular immune responses

Jennifer Schepp-Berglind, Min Luo, Danher Wang, Jason A. Wicker, Nicholas U. Raja, Brian D. Hoel, David H. Holman, Alan D.T. Barrett, John Y. Dong

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

West Nile Virus (WNV), a member of the family Flaviviridae, was first identified in Africa in 1937. In recent years, it has spread into Europe and North America. The clinical manifestations of WNV infection range from mild febrile symptoms to fatal encephalitis. Two genetic lineages (lineages I and II) are recognized; lineage II is associated with mild disease, while lineage I has been associated with severe disease, including encephalitis. WNV has now spread across North America, significantly affecting both public and veterinary health. In the efforts to develop an effective vaccine against all genetic variants of WNV, we have studied the feasibility of inducing both neutralizing and cellular immune responses by de novo synthesis of WNV antigens using a complex adenoviral vaccine (CAdVax) vector. By expressing multiple WNV proteins from a single vaccine vector, we were able to induce both humoral and cellular immune responses in vaccinated mice. Neutralization assays demonstrated that the antibodies were broadly neutralizing against both lineages of WNV, with a significant preference for the homologous lineage II virus. The results from this study show that multiple antigens synthesized de novo from a CAdVax vector are capable of inducing both humoral and cellular immune responses against WNV and that a multiantigen approach may provide broad protection against multiple genetic variants of WNV.

Original languageEnglish (US)
Pages (from-to)1117-1126
Number of pages10
JournalClinical and Vaccine Immunology
Volume14
Issue number9
DOIs
StatePublished - Sep 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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