Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range

Heather M. Callaway, Kathrin Welsch, Wendy Weichert, Andrew B. Allison, Susan L. Hafenstein, Kai Huang, Sho Iketani, Colin R. Parrish

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Antibody and receptor binding are key virus-host interactions that control host range and determine the success of infection. Canine and feline parvovirus capsids bind the transferrin receptor type 1 (TfR) to enter host cells, and specific structural interactions appear necessary to prepare the stable capsids for infection. Here, we define the details of binding, competition, and occupancy of wild-type and mutant parvovirus capsids with purified receptors and antibodies. TfR-capsid binding interactions depended on the TfR species and varied widely, with no direct relationship between binding affinity and infection. Capsids bound feline, raccoon, and black-backed jackal TfRs at high affinity but barely bound canine TfRs, which mediated infection efficiently. TfRs from different species also occupied capsids to different levels, with an estimated 1 to 2 feline TfRs but 12 black-backed jackal TfRs binding each capsid. Multiple alanine substitutions within loop 1 on the capsid surface reduced TfR binding but substitutions within loop 3 did not, suggesting that loop 1 directly engaged the TfR and loop 3 sterically affected that interaction. Binding and competition between different TfRs and/or antibodies showed complex relationships. Both antibodies 14 and E competed capsids off TfRs, but antibody E could also compete capsids off itself and antibody 14, likely by inducing capsid structural changes. In some cases, the initial TfR or antibody binding event affected subsequent TfR binding, suggesting that capsid structure changes occur after TfR or antibody binding and may impact infection. This shows that precise, host-specific TfR-capsid interactions, beyond simple attachment, are important for successful infection.

Original languageEnglish (US)
Article numbere00460-18
JournalJournal of Virology
Volume92
Issue number13
DOIs
StatePublished - Jul 1 2018

Fingerprint

Parvovirus
Protoparvovirus
Transferrin Receptors
capsid
Capsid
Host Specificity
transferrin
Infection Control
host range
receptors
antibodies
Antibodies
infection
cells
Jackals
Infection
jackals
Felidae
Feline Panleukopenia Virus
Feline parvovirus

Keywords

  • Antibody competition
  • Bio-layer interferometry
  • Canine parvovirus
  • Conformational change
  • Feline panleukopenia virus
  • Infection
  • Neutralization
  • Receptor binding
  • Transferrin receptor

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range. / Callaway, Heather M.; Welsch, Kathrin; Weichert, Wendy; Allison, Andrew B.; Hafenstein, Susan L.; Huang, Kai; Iketani, Sho; Parrish, Colin R.

In: Journal of Virology, Vol. 92, No. 13, e00460-18, 01.07.2018.

Research output: Contribution to journalArticle

Callaway, Heather M. ; Welsch, Kathrin ; Weichert, Wendy ; Allison, Andrew B. ; Hafenstein, Susan L. ; Huang, Kai ; Iketani, Sho ; Parrish, Colin R. / Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range. In: Journal of Virology. 2018 ; Vol. 92, No. 13.
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