Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova, Anton V. Liopo, Valery G. Andreev, Inga Cicenaite, B. Mark Evers, Shilla Chakrabarty, Todd C. Pappas, Rinat O. Esenaliev

Research output: Contribution to journalArticle

126 Scopus citations

Abstract

The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalCancer Letters
Volume261
Issue number2
DOIs
StatePublished - Mar 18 2008

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Keywords

  • Gene delivery
  • PEI
  • PLGA nanoparticles
  • Tumor
  • Ultrasound

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Chumakova, O. V., Liopo, A. V., Andreev, V. G., Cicenaite, I., Evers, B. M., Chakrabarty, S., Pappas, T. C., & Esenaliev, R. O. (2008). Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo. Cancer Letters, 261(2), 215-225. https://doi.org/10.1016/j.canlet.2007.11.023