Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova; Anton V. Liopo; Valery G. Andreev; Inga Cicenaite; B. Mark Evers; Shilla Chakrabarty; Todd C. Pappas; Rinat O. Esenaliev

doi:10.1016/j.canlet.2007.11.023

Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Olga V. Chumakova
, Anton V. Liopo
, Valery G. Andreev
, Inga Cicenaite
, B. Mark Evers
, Shilla Chakrabarty
, Todd C. Pappas
, Rinat O. Esenaliev

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

Original language	English (US)
Pages (from-to)	215-225
Number of pages	11
Journal	Cancer Letters
Volume	261
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2007.11.023
State	Published - Mar 18 2008

Keywords

Gene delivery
PEI
PLGA nanoparticles
Tumor
Ultrasound

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2007.11.023

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title = "Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo",

abstract = "The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.",

keywords = "Gene delivery, PEI, PLGA nanoparticles, Tumor, Ultrasound",

author = "Chumakova, \{Olga V.\} and Liopo, \{Anton V.\} and Andreev, \{Valery G.\} and Inga Cicenaite and Evers, \{B. Mark\} and Shilla Chakrabarty and Pappas, \{Todd C.\} and Esenaliev, \{Rinat O.\}",

note = "Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant \#W81XWH-04-1-0247) and by the NIH Grant \#R01CA104748. ",

year = "2008",

month = mar,

day = "18",

doi = "10.1016/j.canlet.2007.11.023",

language = "English (US)",

volume = "261",

pages = "215--225",

journal = "Cancer Letters",

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AU - Chumakova, Olga V.

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AU - Andreev, Valery G.

AU - Cicenaite, Inga

AU - Evers, B. Mark

AU - Chakrabarty, Shilla

AU - Pappas, Todd C.

AU - Esenaliev, Rinat O.

N1 - Funding Information: These studies are supported in part by the Department of Defense Prostate Cancer Research Program (Grant #W81XWH-04-1-0247) and by the NIH Grant #R01CA104748.

PY - 2008/3/18

Y1 - 2008/3/18

N2 - The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

AB - The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA β-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of β-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.

KW - Gene delivery

KW - PEI

KW - PLGA nanoparticles

KW - Tumor

KW - Ultrasound

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Composition of PLGA and PEI/DNA nanoparticles improves ultrasound-mediated gene delivery in solid tumors in vivo

Abstract

Keywords

ASJC Scopus subject areas

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