Comprehensive characterization of chondrocyte cultures in plasma and whole blood biomatrices for cartilage tissue engineering

Ronny M. Schulz, Marcus Haberhauer, Göran Zernia, Claudia Pösel, Christian Thümmler, Jeremy Somerson, Daniel Huster

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Many synthetic polymers and biomaterials have been used as matrices for 3D chondrocyte seeding and transplantation in the field of cartilage tissue engineering. To develop a fully autologous carrier for chondrocyte cultivation, we examined the feasibility of allogeneic plasma and whole blood-based matrices and compared them to agarose constructs. Primary articular chondrocytes isolated from 12-month-old pigs were embedded into agarose, plasma and whole blood matrices and cultivated under static-free swelling conditions for up to four weeks. To evaluate the quality of the synthesized extracellular matrix (ECM), constructs were subjected to weekly examinations using histological staining, spectrophotometry, immunohistochemistry and biochemical analysis. In addition, gene expression of cartilage-specific markers such as aggrecan, Sox9 and collagen types I, II and X was determined by RT-PCR. Chondrocyte morphology was assessed via scanning electron microscopy and viability staining, including proliferation and apoptosis assays. Finally, 13C NMR spectroscopy provided further evidence of synthesis of ECM components. It was shown that chondrocyte cultivation in allogeneic plasma and whole-blood matrices promoted sufficient chondrocyte viability and differentiation behaviour, resulting in neo-formation of a hyaline-like cartilage matrix.

Original languageEnglish (US)
Pages (from-to)566-577
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume8
Issue number7
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Cartilage
Tissue Engineering
Chondrocytes
Tissue engineering
Blood
Plasmas
Sepharose
Transplantation (surgical)
Aggrecans
Spectrophotometry
Biocompatible Materials
Cell death
Collagen Type I
Collagen
Gene expression
Biomaterials
Nuclear magnetic resonance spectroscopy
Extracellular Matrix
Swelling
Assays

Keywords

  • Biomatrices
  • Cartilage
  • Chondrocytes
  • Chondroitin sulfate
  • NMR spectroscopy
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)

Cite this

Comprehensive characterization of chondrocyte cultures in plasma and whole blood biomatrices for cartilage tissue engineering. / Schulz, Ronny M.; Haberhauer, Marcus; Zernia, Göran; Pösel, Claudia; Thümmler, Christian; Somerson, Jeremy; Huster, Daniel.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 8, No. 7, 2014, p. 566-577.

Research output: Contribution to journalArticle

Schulz, Ronny M. ; Haberhauer, Marcus ; Zernia, Göran ; Pösel, Claudia ; Thümmler, Christian ; Somerson, Jeremy ; Huster, Daniel. / Comprehensive characterization of chondrocyte cultures in plasma and whole blood biomatrices for cartilage tissue engineering. In: Journal of Tissue Engineering and Regenerative Medicine. 2014 ; Vol. 8, No. 7. pp. 566-577.
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