Compromised Intestinal Barrier Resilience to Disruption in People With HIV

  • Maliha W. Shaikh
  • , Shalini Singh
  • , Leila B. Giron
  • , Lijuan Zhang
  • , Shivanjali Shankaran
  • , Phillip A. Engen
  • , Michelle Villanueva
  • , Ashish Arunkumar Sharma
  • , Rafick Pierre Sekaly
  • , Alan Landay
  • , Ali Keshavarzian
  • , Mohamed Abdel-Mohsen

Research output: Contribution to journalArticlepeer-review

Abstract

People with HIV (PWH) experience chronic intestinal barrier dysfunction, which contributes to chronic inflammation and its comorbidities. The mechanisms underlying this phenomenon remain unclear. We hypothesize that living with HIV compromises the intestinal barrier’s resilience to injurious agents, increasing susceptibility to leakage upon exposure to disruptors. Using intestinal organoids (colonoids and enteroids) derived from PWH and controls, we assessed the impact of alcohol and inflammatory cytokines on barrier integrity. Organoids from PWH exhibited reduced resilience to disruption compared to controls, correlating with alterations in pathways critical to epithelial integrity. These findings reveal a novel mechanism underlying intestinal dysfunction in PWH.

Original languageEnglish (US)
Pages (from-to)364-369
Number of pages6
JournalJournal of Infectious Diseases
Volume232
Issue number2
DOIs
StatePublished - Aug 15 2025

Keywords

  • HIV
  • gut
  • inflammation
  • intestinal barrier
  • microbial translocation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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