TY - JOUR
T1 - Computational Guided Method Applied to LSPR-Based Biosensor for Specific Detection of the Four-Serotypes of Dengue Virus in Seropositive Patients
AU - Machado, Gabriel L.
AU - Teixeira, Felipe M.F.
AU - Ferreira, Gabriel S.C.
AU - Versiani, Alice F.
AU - Andrade, Lidia M.
AU - Ladeira, Luiz O.
AU - da Fonseca, Flávio G.
AU - Ramirez, Jhonattan C.
N1 - Funding Information:
This work received financial support from FAPEMIG (CBB-APQ-03081-17 - Edital 001/2017—Demanda Universal, CBB- APQ-04295-17 - Edital 007/2017 - Programa de Pesquisa para o SUS/PPSUS, Rede Mineira de Pesquisa e Inovação para Bioengenharia de Nanossistemas RM PI-BEM—FAPEMIG - TEC - RED-00282-16), and Federal University of Minas Gerais (UFMG) Edital 03/2019 ADRC. A FINEP-Zika-UFMG Institutional Grant was also used in the development of this work. AFV is supported by a FAPESP Fellow grant (18/17647-0). The authors also thank Dr. Estefânia Mara do Nascimento Martins, from the Centro de Desenvolvimento da Tecnologia Nuclear, CDTN-CNEN, for the measurements made in the Transmitted Electron Microscope.
Funding Information:
This work received financial support from FAPEMIG (CBB‐APQ‐03081‐17 ‐ Edital 001/2017—Demanda Universal, CBB‐ APQ‐04295‐17 ‐ Edital 007/2017 ‐ Programa de Pesquisa para o SUS/PPSUS, Rede Mineira de Pesquisa e Inovação para Bioengenharia de Nanossistemas RM PI‐BEM—FAPEMIG ‐ TEC ‐ RED‐00282‐16), and Federal University of Minas Gerais (UFMG) Edital 03/2019 ADRC. A FINEP‐Zika‐UFMG Institutional Grant was also used in the development of this work. AFV is supported by a FAPESP Fellow grant (18/17647‐0). The authors also thank Dr. Estefânia Mara do Nascimento Martins, from the Centro de Desenvolvimento da Tecnologia Nuclear, CDTN‐CNEN, for the measurements made in the Transmitted Electron Microscope.
Publisher Copyright:
© 2022 Wiley-VCH GmbH
PY - 2022/3
Y1 - 2022/3
N2 - In this paper, a localized surface plasmon resonance (LSPR)-based biosensor, computationally guided to detect selectively the four Dengue virus (DENVx) serotypes in seropositive patients, is presented. The behavior of gold nanoparticles in the shape of nanorods is theoretically and numerically studied as a function of induced structural variations, which are experimentally evidenced due to the bio-interaction between the target analytes and its surface during the detection process. Additionally, with the implementation of the largest Lyapunov exponent, it is possible to calculate the notion of predictability for the experimental results, observing chaotic systems with a very low probability of repetition. Due to the above, when analyzing the recurrence map associated with the obtained resonance curve generated by the LSPR system, the genetic similarity of DENV3/DENV2 and DENV4/DENV1 is evidenced. Finally, the biosensors are validated by analyzing samples of seronegative patients for DENVx and seropositive ones for other Flaviviruses such as Zika virus, Yellow Fever virus, and Saint Louis Encephalitis virus.
AB - In this paper, a localized surface plasmon resonance (LSPR)-based biosensor, computationally guided to detect selectively the four Dengue virus (DENVx) serotypes in seropositive patients, is presented. The behavior of gold nanoparticles in the shape of nanorods is theoretically and numerically studied as a function of induced structural variations, which are experimentally evidenced due to the bio-interaction between the target analytes and its surface during the detection process. Additionally, with the implementation of the largest Lyapunov exponent, it is possible to calculate the notion of predictability for the experimental results, observing chaotic systems with a very low probability of repetition. Due to the above, when analyzing the recurrence map associated with the obtained resonance curve generated by the LSPR system, the genetic similarity of DENV3/DENV2 and DENV4/DENV1 is evidenced. Finally, the biosensors are validated by analyzing samples of seronegative patients for DENVx and seropositive ones for other Flaviviruses such as Zika virus, Yellow Fever virus, and Saint Louis Encephalitis virus.
KW - LSPR biosensors
KW - flavivirus
KW - numerical modeling
KW - plasmonic
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U2 - 10.1002/ppsc.202100157
DO - 10.1002/ppsc.202100157
M3 - Article
AN - SCOPUS:85124452581
SN - 0934-0866
VL - 39
JO - Particle and Particle Systems Characterization
JF - Particle and Particle Systems Characterization
IS - 3
M1 - 2100157
ER -