Computer analysis of mFISH chromosome aberration data uncovers an excess of very complicated metaphases

M. Vazquez, K. M. Greulich-Bode, J. Arsuaga, Michael Cornforth, M. Brückner, R. K. Sachs, L. Hlatky, M. Molls, P. Hahnfeldt

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To analyse spectra of chromosome aberrations induced in vitro by low LET radiation, in order to characterize radiation damage mechanisms quantitatively. Methods: Multiplex fluorescence in situ hybridization (mFISH) allows the simultaneous identification of each homologous chromosome pair by its own colour. mFISH data, specifying number distributions for colour junctions in metaphases of human peripheral blood lymphocytes 72 hours after exposure in vitro to a 3 Gy γ-ray dose, were combined with similar, previously published results. Monte Carlo computer implementations of radiobiological models for chromosome aberration production guided quantitative analyses, which took into account distribution of cells among different metaphases and lethal effects or preferential elimination of some aberrations at cell division. Results and Conclusions: Standard models of DNA damage induction/repair/misrepair explain the main trends of the data as regards the fraction of metaphases having a particular number of colours involved in colour junctions. However, all standard models systematically under-predict the observed fraction of metaphases where a large number of different chromosomes participate in aberrations. An early appearance of chromosomal instability could explain most of the discrepancies.

Original languageEnglish (US)
Pages (from-to)1103-1115
Number of pages13
JournalInternational Journal of Radiation Biology
Volume78
Issue number12
DOIs
StatePublished - Dec 1 2002

Fingerprint

computer analysis
chromosome aberrations
Metaphase
Chromosomes
fluorescence in situ hybridization
Aberrations
Fluorescence In Situ Hybridization
metaphase
Chromosome Aberrations
Color
Fluorescence
color
fluorescence
chromosomes
aberration
Radiation
cell division
Linear Energy Transfer
Chromosomal Instability
Lymphocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Computer analysis of mFISH chromosome aberration data uncovers an excess of very complicated metaphases. / Vazquez, M.; Greulich-Bode, K. M.; Arsuaga, J.; Cornforth, Michael; Brückner, M.; Sachs, R. K.; Hlatky, L.; Molls, M.; Hahnfeldt, P.

In: International Journal of Radiation Biology, Vol. 78, No. 12, 01.12.2002, p. 1103-1115.

Research output: Contribution to journalArticle

Vazquez, M, Greulich-Bode, KM, Arsuaga, J, Cornforth, M, Brückner, M, Sachs, RK, Hlatky, L, Molls, M & Hahnfeldt, P 2002, 'Computer analysis of mFISH chromosome aberration data uncovers an excess of very complicated metaphases', International Journal of Radiation Biology, vol. 78, no. 12, pp. 1103-1115. https://doi.org/10.1080/09553000210166354
Vazquez, M. ; Greulich-Bode, K. M. ; Arsuaga, J. ; Cornforth, Michael ; Brückner, M. ; Sachs, R. K. ; Hlatky, L. ; Molls, M. ; Hahnfeldt, P. / Computer analysis of mFISH chromosome aberration data uncovers an excess of very complicated metaphases. In: International Journal of Radiation Biology. 2002 ; Vol. 78, No. 12. pp. 1103-1115.
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