Computerized image-analysis microspectroscopy of tissue sections

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Abstract

A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.

Original languageEnglish (US)
Pages (from-to)787-790
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume112
Issue number8
StatePublished - Jan 1 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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