Computerized image-analysis microspectroscopy of tissue sections

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.

Original languageEnglish (US)
Pages (from-to)787-790
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume112
Issue number8
StatePublished - 1988
Externally publishedYes

Fingerprint

Subtraction Technique
Computer-Assisted Image Processing
Lighting
Light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Computerized image-analysis microspectroscopy of tissue sections. / Pappolla, Miguel.

In: Archives of Pathology and Laboratory Medicine, Vol. 112, No. 8, 1988, p. 787-790.

Research output: Contribution to journalArticle

@article{e2f861729f5548089d933b2d44efb4c1,
title = "Computerized image-analysis microspectroscopy of tissue sections",
abstract = "A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.",
author = "Miguel Pappolla",
year = "1988",
language = "English (US)",
volume = "112",
pages = "787--790",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "8",

}

TY - JOUR

T1 - Computerized image-analysis microspectroscopy of tissue sections

AU - Pappolla, Miguel

PY - 1988

Y1 - 1988

N2 - A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.

AB - A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.

UR - http://www.scopus.com/inward/record.url?scp=0023735362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023735362&partnerID=8YFLogxK

M3 - Article

VL - 112

SP - 787

EP - 790

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 8

ER -