A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.
|Original language||English (US)|
|Number of pages||4|
|Journal||Archives of Pathology and Laboratory Medicine|
|State||Published - Jan 1 1988|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology