Concentration of membrane antigens by forward transport and trapping in neuronal growth cones

Michael Sheetz, Nikki L. Baumrind, Denise B. Wayne, Alan L. Pearlman

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Formation of the nervous system requires that neuronal growth cones follow specific paths and then stop at recognition signals, sensed at the growth cone's leading edge. We used antibody-coated gold particles viewed by video-enhanced differential interference contrast microscopy to observe the distribution and movement of two cell surface molecules, N-CAM and the 2A1 antigen, on growth cones of cultured cortical neurons. Gold particles are occassionally transported forward at 1-2 μm/s to the leading edge where they are trapped but continue to move. Concentration at the edge persists after cytochalasin D treatment or ATP depletion, but active movements to and along edges cease. We also observed a novel outward movement of small cytoplasmic aggregates at 1.8 μm/s in filopodia. We suggest that active forward transport and trapping involve reversible attachment of antigens to and transport along cytoskeletal elements localized to edges of growth cones.

Original languageEnglish (US)
Pages (from-to)231-241
Number of pages11
JournalCell
Volume61
Issue number2
DOIs
StatePublished - Apr 20 1990
Externally publishedYes

Fingerprint

Growth Cones
Cones
Membranes
Antigens
Gold
Interference Microscopy
Cytochalasin D
Pseudopodia
Active Biological Transport
Neurology
Computer aided manufacturing
Nervous System
Neurons
Cell Movement
Microscopic examination
Adenosine Triphosphate
Molecules
Antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Concentration of membrane antigens by forward transport and trapping in neuronal growth cones. / Sheetz, Michael; Baumrind, Nikki L.; Wayne, Denise B.; Pearlman, Alan L.

In: Cell, Vol. 61, No. 2, 20.04.1990, p. 231-241.

Research output: Contribution to journalArticle

Sheetz, Michael ; Baumrind, Nikki L. ; Wayne, Denise B. ; Pearlman, Alan L. / Concentration of membrane antigens by forward transport and trapping in neuronal growth cones. In: Cell. 1990 ; Vol. 61, No. 2. pp. 231-241.
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