Concurrent cytogenetic, interphase fluorescence in situ hybridization and DNA flow cytometric analyses of a carcinoma ex-pleomorphic adenoma of parotid gland

Adel K. El-Naggar, Mercedes Lovell, David L. Callender, Nelson G. Ordonez, Ann M. Killary

    Research output: Contribution to journalArticle

    8 Scopus citations

    Abstract

    We report the cytogenetic, fluorescence in situ hybridization (FISH), and DNA ploidy analyses of a high grade carcinoma ex-pleomorphic adenoma of the submandibular gland. Our overall combined analyses showed a marked DNA aneuploidy and numerical abnormalities involving all chromosomes. Cytogenetic analysis revealed a near tetraploid modal chromosomal number with tetraploid loss of chromosomes Y, 1, 6, 9, 11, 14, 15, 17, and 19-21 and hypertetraploid gain of chromosomes 7, 8, and 22. The structural abnormalities included der(1;14)(q10;q10),del(6)(q15q34),+del(6)(q15q34),+der(8)t(1;8)(q12;q12.2),de r(9;19)(q10;q10),add(14)(p11.2),i(20)(q10),der(21)t(8;21)(q11.2;q22.3), +der(21)t(8;21)(q11.2;q22.3). Interphase FISH of the primary and short-term cultured cells using directly labeled pericentromeric probes for chromosomes 6-12, 17, 18, and Y resulted in alterations corresponding to the cytogenetic findings. DNA ploidy analysis of both the primary and cultured tumor cells showed a hyperdiploid stemline with DNA indices of 2.6. The results indicate that: (1) marked numerical, structural chromosomal, and DNA content abnormalities are present in this tumor; and (2) alteration at 8q and 6q regions, together with previous results, suggest an association between these events and the development and/or progression of this tumor.

    Original languageEnglish (US)
    Pages (from-to)132-136
    Number of pages5
    JournalCancer Genetics and Cytogenetics
    Volume107
    Issue number2
    DOIs
    StatePublished - Dec 1 1998

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Cancer Research

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