Concurrent neuroborreliosis and alzheimer's disease

Analysis of the evidence

Miguel Pappolla, R. Omar, B. Saran, A. Andorn, M. Suarez, C. Pavia, A. Weinstein, D. Shank, K. Davis, W. Burgdorfer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Several recent reports have claimed a possible association between Borrelia burgdorferi infection and Alzheimer's disease (AD). Herein, we describe our search for additional evidence of neuroborreliosis in AD. Brain tissue from neuropathologically confirmed cases of AD was cultured for B burgdorferi using standard microbiologic methods. Material derived from culture was further examined using electron microscopy, direct immunofluorescence, and acridine orange fluorescence. Previous studies have shown high titers of antiborrelia antibodies in CSF in all cases of confirmed neuroborreliosis; therefore, we tested CSF from neuropathologically confirmed cases of AD by indirect immunofluorescence and enzyme-linked immunoassay. In addition, imprint preparations from AD and control brain tissues were studied by direct immunofluorescence using a monoclonal antiborrelia antibody. Finally, a Western blot method was used to analyze protein extracts from cultures and AD brain tissue for the presence of borrelia antigen. Contrary to previous studies, our results do not support an association between infection with B burgdorferi and AD.

Original languageEnglish (US)
Pages (from-to)753-757
Number of pages5
JournalHuman Pathology
Volume20
Issue number8
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Alzheimer Disease
Direct Fluorescent Antibody Technique
Brain
Borrelia Infections
Borrelia
Acridine Orange
Borrelia burgdorferi
Indirect Fluorescent Antibody Technique
Immunoenzyme Techniques
Electron Microscopy
Fluorescence
Western Blotting
Monoclonal Antibodies
Antigens
Antibodies
Infection
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Pappolla, M., Omar, R., Saran, B., Andorn, A., Suarez, M., Pavia, C., ... Burgdorfer, W. (1989). Concurrent neuroborreliosis and alzheimer's disease: Analysis of the evidence. Human Pathology, 20(8), 753-757. https://doi.org/10.1016/0046-8177(89)90068-3

Concurrent neuroborreliosis and alzheimer's disease : Analysis of the evidence. / Pappolla, Miguel; Omar, R.; Saran, B.; Andorn, A.; Suarez, M.; Pavia, C.; Weinstein, A.; Shank, D.; Davis, K.; Burgdorfer, W.

In: Human Pathology, Vol. 20, No. 8, 1989, p. 753-757.

Research output: Contribution to journalArticle

Pappolla, M, Omar, R, Saran, B, Andorn, A, Suarez, M, Pavia, C, Weinstein, A, Shank, D, Davis, K & Burgdorfer, W 1989, 'Concurrent neuroborreliosis and alzheimer's disease: Analysis of the evidence', Human Pathology, vol. 20, no. 8, pp. 753-757. https://doi.org/10.1016/0046-8177(89)90068-3
Pappolla, Miguel ; Omar, R. ; Saran, B. ; Andorn, A. ; Suarez, M. ; Pavia, C. ; Weinstein, A. ; Shank, D. ; Davis, K. ; Burgdorfer, W. / Concurrent neuroborreliosis and alzheimer's disease : Analysis of the evidence. In: Human Pathology. 1989 ; Vol. 20, No. 8. pp. 753-757.
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