Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers

Rakez Kayed, Isabel Canto, Leonid Breydo, Suhail Rasool, Tamas Lukacsovich, Jessica Wu, Ricardo Albay, Anna Pensalfini, Stephen Yeung, Elizabeth Head, J. Lawrence Marsh, Charles Glabe

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Background. Age-related neurodegenerative diseases share a number of important pathological features, such as accumulation of misfolded proteins as amyloid oligomers and fibrils. Recent evidence suggests that soluble amyloid oligomers and not the insoluble amyloid fibrils may represent the primary pathological species of protein aggregates. Results. We have produced several monoclonal antibodies that specifically recognize prefibrillar oligomers and do not recognize amyloid fibrils, monomer or natively folded proteins. Like the polyclonal antisera, the individual monoclonals recognize generic epitopes that do not depend on a specific linear amino acid sequence, but they display distinct preferences for different subsets of prefibrillar oligomers. Immunological analysis of a number of different prefibrillar A oligomer preparations show that structural polymorphisms exist in A prefibrillar oligomers that can be distinguished on the basis of their reactivity with monoclonal antibodies. Western blot analysis demonstrates that the conformers defined by the monoclonal antibodies have distinct size distributions, indicating that oligomer structure varies with size. The different conformational types of A prefibrillar oligomers can serve as they serve as templates for monomer addition, indicating that they seed the conversion of A monomer into more prefibrillar oligomers of the same type. Conclusions. These results indicate that distinct structural variants or conformers of prefibrillar A oligomers exist that are capable of seeding their own replication. These conformers may be analogous to different strains of prions.

Original languageEnglish (US)
Article number57
JournalMolecular Neurodegeneration
Volume5
Issue number1
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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    Kayed, R., Canto, I., Breydo, L., Rasool, S., Lukacsovich, T., Wu, J., Albay, R., Pensalfini, A., Yeung, S., Head, E., Marsh, J. L., & Glabe, C. (2010). Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers. Molecular Neurodegeneration, 5(1), [57]. https://doi.org/10.1186/1750-1326-5-57