Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy

Peter Schultze, Erich Wörgötter, Werner Braun, Gerhard Wagner, Milan Vašák, Jeremias H R Kägi, Kurt Wüthrich

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints on the geometry of the metal-cysteine clusters determined by heteronuclear correlation spectroscopy, and dihedral angle constraints derived from both coupling constants and nuclear Overhauser effects. The structure calculations were performed with the program DISMAN. As in previous studies with rabbit liver metallothionein-2a, the structure calculations were performed separately for the α and β-domains containing the 4 and 3-metal clusters, respectively, since no interdomain constraints were found. For both domains, the global polypeptide fold, the location of polypeptide secondary structure elements, the architecture of the metal-sulfur cluster and the local chirality of the metal co-ordination are very similar to the solution structure of rabbit metallothionein-2a, but show considerable difference relative to the crystal structure of rat metallothionein-2.

Original languageEnglish (US)
Pages (from-to)251-268
Number of pages18
JournalJournal of Molecular Biology
Volume203
Issue number1
DOIs
StatePublished - Sep 5 1988
Externally publishedYes

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Metallothionein
Magnetic Resonance Spectroscopy
Metals
Liver
Protons
Spectrum Analysis
Rabbits
Peptides
Sulfur
Cysteine

ASJC Scopus subject areas

  • Virology

Cite this

Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy. / Schultze, Peter; Wörgötter, Erich; Braun, Werner; Wagner, Gerhard; Vašák, Milan; Kägi, Jeremias H R; Wüthrich, Kurt.

In: Journal of Molecular Biology, Vol. 203, No. 1, 05.09.1988, p. 251-268.

Research output: Contribution to journalArticle

Schultze, Peter ; Wörgötter, Erich ; Braun, Werner ; Wagner, Gerhard ; Vašák, Milan ; Kägi, Jeremias H R ; Wüthrich, Kurt. / Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy. In: Journal of Molecular Biology. 1988 ; Vol. 203, No. 1. pp. 251-268.
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