Conformational states governing the rates of spontaneous transition mutations

R. Mitra, Bernard Pettitt, R. D. Blake

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We report the results of a theoretical study, combining the results of sequence analysis and integral equation structural methods for nucleic acids in aqueous solutions, on the effects of nearest neighbors on the (T-G) mispair in solution for 12 nearest neighbor contexts. Attempts have been made to classify the structural and energetic effects of the 5' and 3' neighbors with respect to the observed spontaneous mutation rates in vertebrates. It is found that 5' nearest neighbor is probably the most critical structural factor in facilitating or discouraging mutations. Local conformational states correlate with discrimination of bases to be excised in mispairs. Our study confirms the role of the flexibility of the DNA molecule in governing the rates of spontaneous mutation.

Original languageEnglish (US)
Pages (from-to)169-179
Number of pages11
JournalBiopolymers
Volume36
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

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