Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174

Marc Morais, Megan Fisher, Shuji Kanamaru, Laralynne Przybyla, John Burgner, Bentley A. Fane, Michael G. Rossmann

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The three-dimensional structure of bacteriophage φX174 external scaffolding protein D, prior to its interaction with other structural proteins, has been determined to 3.3 Å by X-ray crystallography. The crystals belong to space group P41212 with a dimer in the asymmetric unit that closely resembles asymmetric dimers observed in the φX174 procapsid structure. Furthermore, application of the crystallographic 41 symmetry operation to one of these dimers generates a tetramer similar to the tetramer in the icosahedral asymmetric unit of the procapsid. These data suggest that both dimers and tetramers of the D protein are true morphogenetic intermediates and can form independently of other proteins involved in procapsid morphogenesis. The crystal structure of the D scaffolding protein thus represents the state of the polypeptide prior to procapsid assembly. Hence, comparison with the procapsid structure provides a rare opportunity to follow the conformational switching events necessary for the construction of complex macromolecular assemblies.

Original languageEnglish
Pages (from-to)991-997
Number of pages7
JournalMolecular Cell
Volume15
Issue number6
DOIs
StatePublished - Sep 24 2004
Externally publishedYes

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Bacteriophages
Proteins
Macromolecular Substances
X Ray Crystallography
Morphogenesis
Peptides

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Morais, M., Fisher, M., Kanamaru, S., Przybyla, L., Burgner, J., Fane, B. A., & Rossmann, M. G. (2004). Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174. Molecular Cell, 15(6), 991-997. https://doi.org/10.1016/j.molcel.2004.08.023

Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174. / Morais, Marc; Fisher, Megan; Kanamaru, Shuji; Przybyla, Laralynne; Burgner, John; Fane, Bentley A.; Rossmann, Michael G.

In: Molecular Cell, Vol. 15, No. 6, 24.09.2004, p. 991-997.

Research output: Contribution to journalArticle

Morais, M, Fisher, M, Kanamaru, S, Przybyla, L, Burgner, J, Fane, BA & Rossmann, MG 2004, 'Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174', Molecular Cell, vol. 15, no. 6, pp. 991-997. https://doi.org/10.1016/j.molcel.2004.08.023
Morais, Marc ; Fisher, Megan ; Kanamaru, Shuji ; Przybyla, Laralynne ; Burgner, John ; Fane, Bentley A. ; Rossmann, Michael G. / Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174. In: Molecular Cell. 2004 ; Vol. 15, No. 6. pp. 991-997.
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