Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques

P. E. Marszalek, A. F. Oberhauser, M. Carrion-Vazquez, J. M. Fernandez

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We use single molecule AFM techniques to trigger mechanical unfolding and mechanical unfolding intermediates in the immunoglobulin modules of an engineered polyprotein. Site directed mutagenesis of the immunoglobulin modules results in well defined mechanical phenotypes that are readily detectable with single molecule AFM techniques. In addition to examining the conformational changes of proteins, we can also use similar techniques to trigger chair to boat, and chair inversion transitions in the pyranose rings of single polysaccharide molecules. Our results demonstrate the use of an AFM to detect conformational changes in single molecules with a resolution similar to that of NMR or X-ray diffraction techniques.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Fingerprint Dive into the research topics of 'Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques'. Together they form a unique fingerprint.

  • Cite this

    Marszalek, P. E., Oberhauser, A. F., Carrion-Vazquez, M., & Fernandez, J. M. (1999). Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1). IEEE.