Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques

P. E. Marszalek, Andres Oberhauser, M. Carrion-Vazquez, J. M. Fernandez

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We use single molecule AFM techniques to trigger mechanical unfolding and mechanical unfolding intermediates in the immunoglobulin modules of an engineered polyprotein. Site directed mutagenesis of the immunoglobulin modules results in well defined mechanical phenotypes that are readily detectable with single molecule AFM techniques. In addition to examining the conformational changes of proteins, we can also use similar techniques to trigger chair to boat, and chair inversion transitions in the pyranose rings of single polysaccharide molecules. Our results demonstrate the use of an AFM to detect conformational changes in single molecules with a resolution similar to that of NMR or X-ray diffraction techniques.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages78
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Polysaccharides
Proteins
Molecules
Immunoglobulins
Polyproteins
Mutagenesis
Boats
Nuclear magnetic resonance
X ray diffraction

ASJC Scopus subject areas

  • Bioengineering

Cite this

Marszalek, P. E., Oberhauser, A., Carrion-Vazquez, M., & Fernandez, J. M. (1999). Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 78). Piscataway, NJ, United States: IEEE.

Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. / Marszalek, P. E.; Oberhauser, Andres; Carrion-Vazquez, M.; Fernandez, J. M.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 78.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Marszalek, PE, Oberhauser, A, Carrion-Vazquez, M & Fernandez, JM 1999, Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 78, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Marszalek PE, Oberhauser A, Carrion-Vazquez M, Fernandez JM. Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 78
Marszalek, P. E. ; Oberhauser, Andres ; Carrion-Vazquez, M. ; Fernandez, J. M. / Conformational transitions in single protein and polysaccharide molecules studied with AFM techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 78
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