Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein

Eric Wagner, Jason K. Ospina, Yue Hu, Miroslav Dundr, A. Gregory Matera, William F. Marzluff

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Formation of the 3′ end of replication-dependent histone mRNAs is most robust during S phase and is mediated by both the stem-loop binding protein (SLBP) and the U7 snRNP. We previously identified a 100-kDa zinc finger protein (ZFP100) as a component of U7 snRNP that interacts with the SLBP/pre-mRNA complex. Here, we show that myc- or GFP-tagged ZFP100 overexpressed after transfection is concentrated in Cajal bodies (CBs), and unlike components of the spliceosomal snRNPs, photobleaching experiments demonstrate that ZFP100 is stably associated with CBs. Of the 18 zinc fingers contained within ZFP100, the region encompassing fingers 2-6 is sufficient to maintain CB localization. Zn fingers 5-10 are required for maximal binding of ZFP100 to a 20-amino-acid region of Lsm11, a U7 snRNP core protein. Expression of ZFP100 stimulates histone mRNA processing in vivo, assayed by activation of a reporter gene that encodes a GFP mRNA ending in a histone 3′ end. Importantly, the domain that is required for CB localization and Lsm11 binding is also sufficient to stimulate histone pre-mRNA processing in vivo. Comparisons with other mammalian ZFP100 orthologs show that the central Zn fingers sufficient for in vivo activity are most highly conserved, whereas the number and sequence of the Zn fingers in the N- and C-terminal domains vary.

Original languageEnglish (US)
Pages (from-to)1206-1218
Number of pages13
JournalRNA
Volume12
Issue number7
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

U7 Small Nuclear Ribonucleoproteins
Zinc Fingers
Histones
RNA Precursors
Messenger RNA
Carrier Proteins
Proteins
snRNP Core Proteins
Photobleaching
S Phase
Reporter Genes
Fingers
Transfection
Amino Acids

Keywords

  • Cajal body
  • Cell cycle
  • Histone mRNA
  • SLBP

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Wagner, E., Ospina, J. K., Hu, Y., Dundr, M., Matera, A. G., & Marzluff, W. F. (2006). Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. RNA, 12(7), 1206-1218. https://doi.org/10.1261/rna.2606

Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. / Wagner, Eric; Ospina, Jason K.; Hu, Yue; Dundr, Miroslav; Matera, A. Gregory; Marzluff, William F.

In: RNA, Vol. 12, No. 7, 2006, p. 1206-1218.

Research output: Contribution to journalArticle

Wagner, E, Ospina, JK, Hu, Y, Dundr, M, Matera, AG & Marzluff, WF 2006, 'Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein', RNA, vol. 12, no. 7, pp. 1206-1218. https://doi.org/10.1261/rna.2606
Wagner, Eric ; Ospina, Jason K. ; Hu, Yue ; Dundr, Miroslav ; Matera, A. Gregory ; Marzluff, William F. / Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. In: RNA. 2006 ; Vol. 12, No. 7. pp. 1206-1218.
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