Construction and preliminary analysis of a metagenomic library from a deep-sea sediment of east Pacific Nodule Province

Meixiang Xu, Fengping Wang, Jun Meng, Xiang Xiao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Pacific Nodule Province is a unique ocean area containing an abundance of polymetallic nodules. To explore more genetic information and discover potentially industrial useful genes of the microbial community from this particular area, a cosmid library with an average insert of about 35 kb was constructed from the deep-sea sediment. The bacteria in the cosmid library were composed mainly of Proteobacteria including Alphaproteobacteria, Gammaproteobacteria and Deltaproteobacteria. The end sequences of some cosmid clones were determined and the complete insert sequences of two cosmid clones, 10D02 and 17H9, are presented. 10D02 has a length of 40.8 kb and contains 40 predicted encoding genes. It contains a partial 16S rRNA gene of Alphaproteobacteria. 17H9 is 36.8 kb and predicted to have 31 encoding genes and a 16S-23S-5S rRNA gene operon. Phylogenetic analysis of 16S and 23S rRNA gene sequence on the 17H9 both reveals that the inserted DNA from 17H9 came from a novel Alphaproteobacteria and is closely related to Magnetospirillum species. The predicted proteins of ORF 1-11 also have high identity to those of Magnetospirillum species, and the organization of these genes is highly conserved among known Magnetospirillum species. The data suggest that the retrieved DNA in 17H9 might be derived from a novel Magnetospirillum species.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalFEMS Microbiology Ecology
Volume62
Issue number3
DOIs
StatePublished - Dec 2007
Externally publishedYes

Fingerprint

Magnetospirillum
Metagenomics
Cosmids
deep-sea sediment
Alphaproteobacteria
Oceans and Seas
rRNA Genes
gene
Microbial Genes
Clone Cells
rRNA Operon
Deltaproteobacteria
Genes
Gammaproteobacteria
Proteobacteria
DNA
clone
Open Reading Frames
Bacteria
analysis

Keywords

  • Deep-sea sediment
  • Magnetospirillum
  • Metagenomic library
  • Microbial diversity
  • Nodule Province

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Construction and preliminary analysis of a metagenomic library from a deep-sea sediment of east Pacific Nodule Province. / Xu, Meixiang; Wang, Fengping; Meng, Jun; Xiao, Xiang.

In: FEMS Microbiology Ecology, Vol. 62, No. 3, 12.2007, p. 233-241.

Research output: Contribution to journalArticle

Xu, Meixiang ; Wang, Fengping ; Meng, Jun ; Xiao, Xiang. / Construction and preliminary analysis of a metagenomic library from a deep-sea sediment of east Pacific Nodule Province. In: FEMS Microbiology Ecology. 2007 ; Vol. 62, No. 3. pp. 233-241.
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