Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Konstantin V. Pugachev; Farshad Guirakhoo; Fred Mitchell; Simeon W. Ocran; Megan Parsons; Barbara W. Johnson; Olga L. Kosoy; Robert S. Lanciotti; John T. Roehrig; Dennis W. Trent; Thomas P. Monath

doi:10.4269/ajtmh.2004.71.639

Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Konstantin V. Pugachev
, Farshad Guirakhoo
, Fred Mitchell
, Simeon W. Ocran
, Megan Parsons
, Barbara W. Johnson
, Olga L. Kosoy
, Robert S. Lanciotti
, John T. Roehrig
, Dennis W. Trent
, Thomas P. Monath

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.

Original language	English (US)
Pages (from-to)	639-645
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	71
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.2004.71.639
State	Published - Nov 2004
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Virology
Infectious Diseases

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10.4269/ajtmh.2004.71.639

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Pugachev, K. V., Guirakhoo, F., Mitchell, F., Ocran, S. W., Parsons, M., Johnson, B. W., Kosoy, O. L., Lanciotti, R. S., Roehrig, J. T., Trent, D. W., & Monath, T. P. (2004). Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool. American Journal of Tropical Medicine and Hygiene, 71(5), 639-645. https://doi.org/10.4269/ajtmh.2004.71.639

Pugachev, KV, Guirakhoo, F, Mitchell, F, Ocran, SW, Parsons, M, Johnson, BW, Kosoy, OL, Lanciotti, RS, Roehrig, JT, Trent, DW & Monath, TP 2004, 'Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool', American Journal of Tropical Medicine and Hygiene, vol. 71, no. 5, pp. 639-645. https://doi.org/10.4269/ajtmh.2004.71.639

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title = "Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool",

abstract = "St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.",

author = "Pugachev, \{Konstantin V.\} and Farshad Guirakhoo and Fred Mitchell and Ocran, \{Simeon W.\} and Megan Parsons and Johnson, \{Barbara W.\} and Kosoy, \{Olga L.\} and Lanciotti, \{Robert S.\} and Roehrig, \{John T.\} and Trent, \{Dennis W.\} and Monath, \{Thomas P.\}",

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doi = "10.4269/ajtmh.2004.71.639",

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AU - Guirakhoo, Farshad

AU - Mitchell, Fred

AU - Ocran, Simeon W.

AU - Parsons, Megan

AU - Johnson, Barbara W.

AU - Kosoy, Olga L.

AU - Lanciotti, Robert S.

AU - Roehrig, John T.

AU - Trent, Dennis W.

AU - Monath, Thomas P.

PY - 2004/11

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AB - St. Louis encephalitis (SLE) and West Nile (WN) flaviviruses are genetically closely related and co-circulate in the United States. Virus neutralization tests provide the most specific means for serodiagnosis of infections with these viruses. However, use of wild-type SLE and WN viral strains for laboratory testing is constrained by the biocontainment requirements. We constructed two highly attenuated yellow fever (YF) virus chimeras that contain the premembrane-envelope (prM-E) protein genes from the virulent MSI-7 (isolated in the United States) or the naturally attenuated CorAn9124 (Argentina) SLE strains. The YF/SLE (CorAn version) virus and the previously constructed YF/WN chimera were shown to specifically distinguish between confirmed human SLE and WN cases in a virus neutralization test using patient sera. These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN.

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Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic tool

Abstract

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