Contained indirect viable cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses

M. W. Cloyd, D. D. Bigner

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A microtechnique for an indirect viable cell membrane immunofluorescence titration assay was developed using Friend murine leukemia virus (F MuLV) producing cells and nonspecific rabbit antisera to F MuLV structural antigens. The assay was sensitive and displayed little variation within or between assays. Since moderate risk tumor viruses, such as recently discovered primate oncornaviruses or feline leukemia virus (FeLV), may be hazardous to laboratory personnel, the assay was adapted for containment of cells infected with such viruses. Cells producing gibbon ape lymphoma virus of FeLV were grown in class III containment cabinets and transferred in sealed flasks to a class II laminar flow cabinet, where the assay was performed. This micromethod not only conserved reagents but also minimized the numbers of moderate risk tumor virus infected cells handled at one time. Centrifugation was contained using custom made devices shown to form a gas tight seal over microtiter plates. Interspecies reactivity of monospecific rabbit antisera against F MuLV structural antigen gp71, but not against p12, was demonstrated for surface antigens on FeLV producing cells.

Original languageEnglish (US)
Pages (from-to)86-90
Number of pages5
JournalJournal of Clinical Microbiology
Volume5
Issue number1
StatePublished - 1977

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Surface Antigens
Fluorescent Antibody Technique
Feline Leukemia Virus
Friend murine leukemia virus
Cell Membrane
Viruses
Oncogenic Viruses
Immune Sera
Hylobates
Rabbits
Laboratory Personnel
Antigens
Hominidae
Retroviridae
Centrifugation
Primates
Lymphoma
Gases
Equipment and Supplies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Contained indirect viable cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses. / Cloyd, M. W.; Bigner, D. D.

In: Journal of Clinical Microbiology, Vol. 5, No. 1, 1977, p. 86-90.

Research output: Contribution to journalArticle

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