Contractile response of canine gallbladder and sphincter of Oddi to substance P and related peptides in vitro

Yan Shi Guo, Pomila Singh, Guillermo Gomez, Srinivasan Rajaraman, James C. Thompson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Substance P (SP) is a neurotransmitter peptide that is widely distributed in the body. Since SP has been demonstra in the gallbladder (GB) and bile ducts of dogs, it may have a role in biliary motility. The objective of this study was to examine the effect of SP on the GB and sphincter of Oddi (SOD) of dogs in vitro, to evaluate the structure-activity relationship of SP, and to compare the contractile effect of SP with that of cholecystokinin octapeptide (CCK-8) and acetylcholine (Ach). Isolated longitudinal strips of GB and SOD from dogs were suspended in oxygenated Krebs buffer and the isometric tension responses to various doses of CCK-8, Ach, SP, and SP homologs [SP-free acid (SPFA), Octa-SP (O-SP), physalaemin (PHY)] were measured. We found that all the SP homologs, other than SPFA, stimulated GB and SOD contractions in vitro in a dose-dependent manner. The potency of SP and its homologs on GB and SOD was SP ≥PHY > O-SP; SPFA was without effect. CCK-8 was significantly more effective than SP on GB contraction, but unlike SP, CCK had no effect on SOD. The maximum contraction achieved by Ach was 1.3 (SOD) to 2.3 (GB) times greater than that achieved by SP, but the ED50of SP was approximately 100- to 200-fold lower than that of Ach. The contractile effect of SP was partially blocked by 10-5M atropine. We suggest from the above results that contractile effects of SP on the dog GB and SOD are probably mediated through binding to specific SP receptors that require the C-terminal amino group and the C-terminal penta-peptide sequence in order to be most effective.

Original languageEnglish (US)
Pages (from-to)812-817
Number of pages6
JournalDigestive Diseases and Sciences
Volume34
Issue number6
DOIs
StatePublished - Jun 1989

Fingerprint

Sphincter of Oddi
Substance P
Gallbladder
Canidae
Peptides
Sincalide
Acetylcholine
Physalaemin
Dogs
In Vitro Techniques
Acids
Neurokinin-1 Receptors

Keywords

  • acetylcholine
  • cholecystokinin
  • gallbladder
  • motility
  • sphincter of Oddi
  • substance P

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Contractile response of canine gallbladder and sphincter of Oddi to substance P and related peptides in vitro. / Guo, Yan Shi; Singh, Pomila; Gomez, Guillermo; Rajaraman, Srinivasan; Thompson, James C.

In: Digestive Diseases and Sciences, Vol. 34, No. 6, 06.1989, p. 812-817.

Research output: Contribution to journalArticle

Guo, Yan Shi ; Singh, Pomila ; Gomez, Guillermo ; Rajaraman, Srinivasan ; Thompson, James C. / Contractile response of canine gallbladder and sphincter of Oddi to substance P and related peptides in vitro. In: Digestive Diseases and Sciences. 1989 ; Vol. 34, No. 6. pp. 812-817.
@article{4f85ecbdf4104311aeb0e6db65a2f63b,
title = "Contractile response of canine gallbladder and sphincter of Oddi to substance P and related peptides in vitro",
abstract = "Substance P (SP) is a neurotransmitter peptide that is widely distributed in the body. Since SP has been demonstra in the gallbladder (GB) and bile ducts of dogs, it may have a role in biliary motility. The objective of this study was to examine the effect of SP on the GB and sphincter of Oddi (SOD) of dogs in vitro, to evaluate the structure-activity relationship of SP, and to compare the contractile effect of SP with that of cholecystokinin octapeptide (CCK-8) and acetylcholine (Ach). Isolated longitudinal strips of GB and SOD from dogs were suspended in oxygenated Krebs buffer and the isometric tension responses to various doses of CCK-8, Ach, SP, and SP homologs [SP-free acid (SPFA), Octa-SP (O-SP), physalaemin (PHY)] were measured. We found that all the SP homologs, other than SPFA, stimulated GB and SOD contractions in vitro in a dose-dependent manner. The potency of SP and its homologs on GB and SOD was SP ≥PHY > O-SP; SPFA was without effect. CCK-8 was significantly more effective than SP on GB contraction, but unlike SP, CCK had no effect on SOD. The maximum contraction achieved by Ach was 1.3 (SOD) to 2.3 (GB) times greater than that achieved by SP, but the ED50of SP was approximately 100- to 200-fold lower than that of Ach. The contractile effect of SP was partially blocked by 10-5M atropine. We suggest from the above results that contractile effects of SP on the dog GB and SOD are probably mediated through binding to specific SP receptors that require the C-terminal amino group and the C-terminal penta-peptide sequence in order to be most effective.",
keywords = "acetylcholine, cholecystokinin, gallbladder, motility, sphincter of Oddi, substance P",
author = "Guo, {Yan Shi} and Pomila Singh and Guillermo Gomez and Srinivasan Rajaraman and Thompson, {James C.}",
year = "1989",
month = "6",
doi = "10.1007/BF01540263",
language = "English (US)",
volume = "34",
pages = "812--817",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Contractile response of canine gallbladder and sphincter of Oddi to substance P and related peptides in vitro

AU - Guo, Yan Shi

AU - Singh, Pomila

AU - Gomez, Guillermo

AU - Rajaraman, Srinivasan

AU - Thompson, James C.

PY - 1989/6

Y1 - 1989/6

N2 - Substance P (SP) is a neurotransmitter peptide that is widely distributed in the body. Since SP has been demonstra in the gallbladder (GB) and bile ducts of dogs, it may have a role in biliary motility. The objective of this study was to examine the effect of SP on the GB and sphincter of Oddi (SOD) of dogs in vitro, to evaluate the structure-activity relationship of SP, and to compare the contractile effect of SP with that of cholecystokinin octapeptide (CCK-8) and acetylcholine (Ach). Isolated longitudinal strips of GB and SOD from dogs were suspended in oxygenated Krebs buffer and the isometric tension responses to various doses of CCK-8, Ach, SP, and SP homologs [SP-free acid (SPFA), Octa-SP (O-SP), physalaemin (PHY)] were measured. We found that all the SP homologs, other than SPFA, stimulated GB and SOD contractions in vitro in a dose-dependent manner. The potency of SP and its homologs on GB and SOD was SP ≥PHY > O-SP; SPFA was without effect. CCK-8 was significantly more effective than SP on GB contraction, but unlike SP, CCK had no effect on SOD. The maximum contraction achieved by Ach was 1.3 (SOD) to 2.3 (GB) times greater than that achieved by SP, but the ED50of SP was approximately 100- to 200-fold lower than that of Ach. The contractile effect of SP was partially blocked by 10-5M atropine. We suggest from the above results that contractile effects of SP on the dog GB and SOD are probably mediated through binding to specific SP receptors that require the C-terminal amino group and the C-terminal penta-peptide sequence in order to be most effective.

AB - Substance P (SP) is a neurotransmitter peptide that is widely distributed in the body. Since SP has been demonstra in the gallbladder (GB) and bile ducts of dogs, it may have a role in biliary motility. The objective of this study was to examine the effect of SP on the GB and sphincter of Oddi (SOD) of dogs in vitro, to evaluate the structure-activity relationship of SP, and to compare the contractile effect of SP with that of cholecystokinin octapeptide (CCK-8) and acetylcholine (Ach). Isolated longitudinal strips of GB and SOD from dogs were suspended in oxygenated Krebs buffer and the isometric tension responses to various doses of CCK-8, Ach, SP, and SP homologs [SP-free acid (SPFA), Octa-SP (O-SP), physalaemin (PHY)] were measured. We found that all the SP homologs, other than SPFA, stimulated GB and SOD contractions in vitro in a dose-dependent manner. The potency of SP and its homologs on GB and SOD was SP ≥PHY > O-SP; SPFA was without effect. CCK-8 was significantly more effective than SP on GB contraction, but unlike SP, CCK had no effect on SOD. The maximum contraction achieved by Ach was 1.3 (SOD) to 2.3 (GB) times greater than that achieved by SP, but the ED50of SP was approximately 100- to 200-fold lower than that of Ach. The contractile effect of SP was partially blocked by 10-5M atropine. We suggest from the above results that contractile effects of SP on the dog GB and SOD are probably mediated through binding to specific SP receptors that require the C-terminal amino group and the C-terminal penta-peptide sequence in order to be most effective.

KW - acetylcholine

KW - cholecystokinin

KW - gallbladder

KW - motility

KW - sphincter of Oddi

KW - substance P

UR - http://www.scopus.com/inward/record.url?scp=0024364066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024364066&partnerID=8YFLogxK

U2 - 10.1007/BF01540263

DO - 10.1007/BF01540263

M3 - Article

VL - 34

SP - 812

EP - 817

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 6

ER -