Contribution of amino acids and insulin to protein anabolism during meal absorption

Elena Volpi, Paola Lucidi, Guido Cruciani, Francesca Monacchia, Gianpaolo Reboldi, Paolo Brunetti, Geremia B. Bolli, Pierpaolo De Feo

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

The contribution of dietary amino acids and endogenous hyperinsulinemia to prandial protein anabolism still has not been established. To this end, leucine estimates ([1-14C]leucine infusion, plasma α-ketoisocaproic acid [KIC] specific activity [SA] as precursor pool SA) of whole-body protein kinetics and fractional secretory rates (FSRs) of albumin, fibrinogen, antithrombin III, and immunoglobulin G (IgG) were measured in three groups of healthy volunteers during intragastric infusion of water (controls, n = 5), liquid glucose-lipid-amino acid (AA) meal (meal+AA, n = 7), or isocaloric glucose-lipid meal (meal-AA, n = 7) that induced the same insulin response as the meal+AA. The results of this study demonstrate that 1) by increasing (P < 0.01) whole-body protein synthesis and decreasing (P < 0.01) proteolysis, dietary amino acids account for the largest part (~90%) of postprandial protein anabolism; 2) the ingestion of an isocaloric meal deprived of amino acids exerts a modest protein anabolic effect (10% of postprandial protein anabolism) by decreasing amino acid oxidation and increasing (P < 0.01) albumin synthesis; 3) albumin FSR is increased (~20%) by postprandial hyperinsulinemia (meal-AA) and additionally increased (~50%) by amino acid intake (meal+AA); 4) IgG FSR is stimulated (~40%) by amino acids, not by insulin; and 5) fibrinogen and antithrombin III FSR are not regulated by amino acids or insulin.

Original languageEnglish (US)
Pages (from-to)1245-1252
Number of pages8
JournalDiabetes
Volume45
Issue number9
StatePublished - 1996
Externally publishedYes

Fingerprint

Meals
Insulin
Amino Acids
Secretory Rate
Proteins
Albumins
Antithrombin III
Hyperinsulinism
Leucine
Fibrinogen
Immunoglobulin G
Lipids
Anabolic Agents
Glucose
Proteolysis
Healthy Volunteers
Eating
Acids
Water

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Volpi, E., Lucidi, P., Cruciani, G., Monacchia, F., Reboldi, G., Brunetti, P., ... De Feo, P. (1996). Contribution of amino acids and insulin to protein anabolism during meal absorption. Diabetes, 45(9), 1245-1252.

Contribution of amino acids and insulin to protein anabolism during meal absorption. / Volpi, Elena; Lucidi, Paola; Cruciani, Guido; Monacchia, Francesca; Reboldi, Gianpaolo; Brunetti, Paolo; Bolli, Geremia B.; De Feo, Pierpaolo.

In: Diabetes, Vol. 45, No. 9, 1996, p. 1245-1252.

Research output: Contribution to journalArticle

Volpi, E, Lucidi, P, Cruciani, G, Monacchia, F, Reboldi, G, Brunetti, P, Bolli, GB & De Feo, P 1996, 'Contribution of amino acids and insulin to protein anabolism during meal absorption', Diabetes, vol. 45, no. 9, pp. 1245-1252.
Volpi E, Lucidi P, Cruciani G, Monacchia F, Reboldi G, Brunetti P et al. Contribution of amino acids and insulin to protein anabolism during meal absorption. Diabetes. 1996;45(9):1245-1252.
Volpi, Elena ; Lucidi, Paola ; Cruciani, Guido ; Monacchia, Francesca ; Reboldi, Gianpaolo ; Brunetti, Paolo ; Bolli, Geremia B. ; De Feo, Pierpaolo. / Contribution of amino acids and insulin to protein anabolism during meal absorption. In: Diabetes. 1996 ; Vol. 45, No. 9. pp. 1245-1252.
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AU - De Feo, Pierpaolo

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