Contribution of coiled-coil assembly to ca2+/ calmodulin-dependent inactivation of TRPC6 channel and its impacts on FSGS-associated phenotypes

Onur K. Polat, Masatoshi Uno, Terukazu Maruyama, Ha Nam Tran, Kayo Imamura, Chee Fah Wong, Reiko Sakaguchi, Mariko Ariyoshi, Kyohei Itsuki, Jun Ichikawa, Takashi Morii, Masahiro Shirakawa, Ryuji Inoue, Katsuhiko Asanuma, Jochen Reiser, Hidehito Tochio, Yasuo Mori, Masayuki X. Mori

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Background TRPC6 is a nonselective cation channel, and mutations of this gene are associated with FSGS. These mutations are associated with TRPC6 current amplitude amplification and/or delay of the channel inactivation (gain-of-function phenotype). However, the mechanism of the gain-of-function in TRPC6 activity has not yet been clearly solved. Methods We performed electrophysiologic, biochemical, and biophysical experiments to elucidate the molecular mechanism underlying calmodulin (CaM)-mediated Ca2+-dependent inactivation (CDI) of TRPC6. To address the pathophysiologic contribution of CDI, we assessed the actin filament organization in cultured mouse podocytes. Results Both lobes of CaM helped induce CDI. Moreover, CaM binding to the TRPC6 CaM-binding domain (CBD) was Ca2+-dependent and exhibited a 1:2 (CaM/CBD) stoichiometry. The TRPC6 coiled-coil assembly, which brought two CBDs into adequate proximity, was essential for CDI. Deletion of the coiled-coil slowed CDI of TRPC6, indicating that the coiled-coil assembly configures both lobes of CaM binding on two CBDs to induce normal CDI. The FSGS-associated TRPC6 mutations within the coiled-coil severely delayed CDI and often increased TRPC6 current amplitudes. In cultured mouse podocytes, FSGS-associated channels and CaM mutations led to sustained Ca2+ elevations and a disorganized cytoskeleton. Conclusions The gain-of-function mechanism found in FSGS-causing mutations in TRPC6 can be explained by impairments of the CDI, caused by disruptions of TRPC’s coiled-coil assembly which is essential for CaM binding. The resulting excess Ca2+ may contribute to structural damage in the podocytes.

Original languageEnglish (US)
Pages (from-to)1587-1603
Number of pages17
JournalJournal of the American Society of Nephrology
Volume30
Issue number9
DOIs
StatePublished - Sep 2019
Externally publishedYes

ASJC Scopus subject areas

  • Nephrology

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