Contribution of the sympathetic nervous system on the burn-associated impairment of CCL3 production

Hitoshi Takahashi, Makiko Kobayashi, Yasuhiro Tsuda, David Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Previously, we have reported that the susceptibility of burned patients to infectious complications is increased when the production of CC-chemokine ligand 3 (CCL3) is impaired. In this study, the role of the sympathetic nervous system on impaired CCL3 production and antibacterial resistance following burn injuries was investigated. Normal mice were resistant (65% survival) to cecal ligation and puncture (CLP)-induced sepsis, while the same CLP killed 90% of thermally injured mice (TI-mice). However, TI-mice resisted CLP-induced sepsis (60% survival) when they were previously treated with CCL3 or sympathectomized with 6-hydroxydopamine (6-OHDA). Augmentation of host resistance against CLP-induced sepsis by 6-OHDA was abrogated by anti-CCL3 mAb treatment. Norepinephrine (NE) production was increased in circulation of TI-mice, and treatment of TI-mice with 6-OHDA resulted in the inhibition of NE secretion. CCL3 production was impaired in cultures of T cells from TI-mice or normal T cells treated with NE, even when stimulated with anti-CD3 mAb. However, CCL3 was produced by mAb-stimulated T cells from TI-mice previously treated with 6-OHDA. These results indicated that by inhibiting CCL3 production the sympathetic nervous system contributes to the increased susceptibility of TI-mice to sepsis.

Original languageEnglish (US)
Pages (from-to)208-214
Number of pages7
JournalCytokine
Volume29
Issue number5
DOIs
StatePublished - Mar 7 2005

Fingerprint

CC Chemokines
Sympathetic Nervous System
Neurology
Burns
Oxidopamine
Ligands
T-cells
Punctures
Ligation
Sepsis
Norepinephrine
T-Lymphocytes
Survival
Cell culture
Wounds and Injuries
Therapeutics

Keywords

  • CCL3
  • Norepinephrine
  • Thermal injury

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Contribution of the sympathetic nervous system on the burn-associated impairment of CCL3 production. / Takahashi, Hitoshi; Kobayashi, Makiko; Tsuda, Yasuhiro; Herndon, David; Suzuki, Fujio.

In: Cytokine, Vol. 29, No. 5, 07.03.2005, p. 208-214.

Research output: Contribution to journalArticle

Takahashi, Hitoshi ; Kobayashi, Makiko ; Tsuda, Yasuhiro ; Herndon, David ; Suzuki, Fujio. / Contribution of the sympathetic nervous system on the burn-associated impairment of CCL3 production. In: Cytokine. 2005 ; Vol. 29, No. 5. pp. 208-214.
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