Contributions of severe burn and disuse to bone structure and strength in rats

L. A. Baer, X. Wu, J. C. Tou, E. Johnson, Steven Wolf, C. E. Wade

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Burn and disuse results in metabolic and bone changes associated with substantial and sustained bone loss. Such loss can lead to an increased fracture incidence and osteopenia. We studied the independent effects of burn and disuse on bone morphology, composition and strength, and microstructure of the bone alterations 14. days after injury. Sprague-Dawley rats were randomized into four groups: Sham/Ambulatory (SA), Burn/Ambulatory (BA), Sham/Hindlimb Unloaded (SH) and Burn/Hindlimb Unloaded (BH). Burn groups received a 40% total body surface area full-thickness scald burn. Disuse by hindlimb unloading was initiated immediately following injury. Bone turnover was determined in plasma and urine. Femur biomechanical parameters were measured by three-point bending tests and bone microarchitecture was determined by micro-computed tomography (uCT). On day 14, a significant reduction in body mass was observed as a result of burn, disuse and a combination of both. In terms of bone health, disuse alone and in combination affected femur weight, length and bone mineral content. Bending failure energy, an index of femur strength, was significantly reduced in all groups and maximum bending stress was lower when burn and disuse were combined. Osteocalcin was reduced in BA compared to the other groups, indicating influence of burn. The reductions observed in femur weight, BMC, biomechanical parameters and indices of bone formation are primarily responses to the combination of burn and disuse. These results offer insight into bone degradation following severe injury and disuse.

Original languageEnglish (US)
Pages (from-to)644-650
Number of pages7
JournalBone
Volume52
Issue number2
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Fingerprint

Bone and Bones
Femur
Burns
Hindlimb
Wounds and Injuries
Hindlimb Suspension
Weights and Measures
Bone Remodeling
Metabolic Bone Diseases
Body Surface Area
Osteocalcin
Osteogenesis
Bone Density
Sprague Dawley Rats
Tomography
Urine
Incidence
Health

Keywords

  • Femur
  • Hindlimb unloading
  • Mechanical bending
  • Micro-computed tomography
  • Thermal injury

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Contributions of severe burn and disuse to bone structure and strength in rats. / Baer, L. A.; Wu, X.; Tou, J. C.; Johnson, E.; Wolf, Steven; Wade, C. E.

In: Bone, Vol. 52, No. 2, 01.02.2013, p. 644-650.

Research output: Contribution to journalArticle

Baer, LA, Wu, X, Tou, JC, Johnson, E, Wolf, S & Wade, CE 2013, 'Contributions of severe burn and disuse to bone structure and strength in rats', Bone, vol. 52, no. 2, pp. 644-650. https://doi.org/10.1016/j.bone.2012.10.032
Baer, L. A. ; Wu, X. ; Tou, J. C. ; Johnson, E. ; Wolf, Steven ; Wade, C. E. / Contributions of severe burn and disuse to bone structure and strength in rats. In: Bone. 2013 ; Vol. 52, No. 2. pp. 644-650.
@article{8d9b92f6ed6640e08e6d2ea11eb8a7c5,
title = "Contributions of severe burn and disuse to bone structure and strength in rats",
abstract = "Burn and disuse results in metabolic and bone changes associated with substantial and sustained bone loss. Such loss can lead to an increased fracture incidence and osteopenia. We studied the independent effects of burn and disuse on bone morphology, composition and strength, and microstructure of the bone alterations 14. days after injury. Sprague-Dawley rats were randomized into four groups: Sham/Ambulatory (SA), Burn/Ambulatory (BA), Sham/Hindlimb Unloaded (SH) and Burn/Hindlimb Unloaded (BH). Burn groups received a 40{\%} total body surface area full-thickness scald burn. Disuse by hindlimb unloading was initiated immediately following injury. Bone turnover was determined in plasma and urine. Femur biomechanical parameters were measured by three-point bending tests and bone microarchitecture was determined by micro-computed tomography (uCT). On day 14, a significant reduction in body mass was observed as a result of burn, disuse and a combination of both. In terms of bone health, disuse alone and in combination affected femur weight, length and bone mineral content. Bending failure energy, an index of femur strength, was significantly reduced in all groups and maximum bending stress was lower when burn and disuse were combined. Osteocalcin was reduced in BA compared to the other groups, indicating influence of burn. The reductions observed in femur weight, BMC, biomechanical parameters and indices of bone formation are primarily responses to the combination of burn and disuse. These results offer insight into bone degradation following severe injury and disuse.",
keywords = "Femur, Hindlimb unloading, Mechanical bending, Micro-computed tomography, Thermal injury",
author = "Baer, {L. A.} and X. Wu and Tou, {J. C.} and E. Johnson and Steven Wolf and Wade, {C. E.}",
year = "2013",
month = "2",
day = "1",
doi = "10.1016/j.bone.2012.10.032",
language = "English (US)",
volume = "52",
pages = "644--650",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Contributions of severe burn and disuse to bone structure and strength in rats

AU - Baer, L. A.

AU - Wu, X.

AU - Tou, J. C.

AU - Johnson, E.

AU - Wolf, Steven

AU - Wade, C. E.

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Burn and disuse results in metabolic and bone changes associated with substantial and sustained bone loss. Such loss can lead to an increased fracture incidence and osteopenia. We studied the independent effects of burn and disuse on bone morphology, composition and strength, and microstructure of the bone alterations 14. days after injury. Sprague-Dawley rats were randomized into four groups: Sham/Ambulatory (SA), Burn/Ambulatory (BA), Sham/Hindlimb Unloaded (SH) and Burn/Hindlimb Unloaded (BH). Burn groups received a 40% total body surface area full-thickness scald burn. Disuse by hindlimb unloading was initiated immediately following injury. Bone turnover was determined in plasma and urine. Femur biomechanical parameters were measured by three-point bending tests and bone microarchitecture was determined by micro-computed tomography (uCT). On day 14, a significant reduction in body mass was observed as a result of burn, disuse and a combination of both. In terms of bone health, disuse alone and in combination affected femur weight, length and bone mineral content. Bending failure energy, an index of femur strength, was significantly reduced in all groups and maximum bending stress was lower when burn and disuse were combined. Osteocalcin was reduced in BA compared to the other groups, indicating influence of burn. The reductions observed in femur weight, BMC, biomechanical parameters and indices of bone formation are primarily responses to the combination of burn and disuse. These results offer insight into bone degradation following severe injury and disuse.

AB - Burn and disuse results in metabolic and bone changes associated with substantial and sustained bone loss. Such loss can lead to an increased fracture incidence and osteopenia. We studied the independent effects of burn and disuse on bone morphology, composition and strength, and microstructure of the bone alterations 14. days after injury. Sprague-Dawley rats were randomized into four groups: Sham/Ambulatory (SA), Burn/Ambulatory (BA), Sham/Hindlimb Unloaded (SH) and Burn/Hindlimb Unloaded (BH). Burn groups received a 40% total body surface area full-thickness scald burn. Disuse by hindlimb unloading was initiated immediately following injury. Bone turnover was determined in plasma and urine. Femur biomechanical parameters were measured by three-point bending tests and bone microarchitecture was determined by micro-computed tomography (uCT). On day 14, a significant reduction in body mass was observed as a result of burn, disuse and a combination of both. In terms of bone health, disuse alone and in combination affected femur weight, length and bone mineral content. Bending failure energy, an index of femur strength, was significantly reduced in all groups and maximum bending stress was lower when burn and disuse were combined. Osteocalcin was reduced in BA compared to the other groups, indicating influence of burn. The reductions observed in femur weight, BMC, biomechanical parameters and indices of bone formation are primarily responses to the combination of burn and disuse. These results offer insight into bone degradation following severe injury and disuse.

KW - Femur

KW - Hindlimb unloading

KW - Mechanical bending

KW - Micro-computed tomography

KW - Thermal injury

UR - http://www.scopus.com/inward/record.url?scp=84870773064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870773064&partnerID=8YFLogxK

U2 - 10.1016/j.bone.2012.10.032

DO - 10.1016/j.bone.2012.10.032

M3 - Article

C2 - 23142361

AN - SCOPUS:84870773064

VL - 52

SP - 644

EP - 650

JO - Bone

JF - Bone

SN - 8756-3282

IS - 2

ER -