Contributions to selective binding of aromatic amino acid residues to tRNAPhe

Research output: Contribution to journalArticle

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Abstract

The binding of aromatic amino acid amides to two fragments of tRNAPhe has been measured, in order to explore contributions to selective interactions of amino acid residues with tRNAPhe. Measurements of the Wye base fluorescence demonstrate that the isolated anticodon loop of tRNAPhe (15 nucleotide residues) has a binding constant K = 75 M-1 for Phe-amide, which is only slightly lower than that observed previously for the complete tRNAPhe (K = 100 M-1). The hexamer GmAAYA,ψ - possessing most residues of the loop, but without loop structure - shows a further reduced binding constant of 42 M-1. According to these data, part of the selective interaction results from the special loop structure and another part probably from the nature of the Wye base. The particular influence of the loop structure is also demonstrated by binding experiments performed in the presence of Mg2+. As expected, addition of Mg2+ decreases the binding affinity of the aromatic amino acid amides. Moreover, Mg2+ at concentrations ≥ 0.3 mM induces cooperative binding of the amides to the anticodon loop similar to that found previously for the complete tRNAPhe, whereas the hexamer does not show any indication for cooperativity. A special coupling of Mg2+ and amide binding to the anticodon loop is also indicated by inhibition of Mg2+ binding in the presence of amides, which is much stronger than expected for a simple salt effect. These results demonstrate a complex coupling of Mg2+ and amide binding to different conformational states of the anticodon loop, which resembles an allosteric type of reaction mechanism.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalBiophysical Chemistry
Volume30
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

RNA, Transfer, Phe
Aromatic Amino Acids
Amides
amino acids
Anticodon
amides
nucleotides
Nucleotides
Salts
Fluorescence
affinity
Amino Acids
indication
fragments
interactions
salts

Keywords

  • Amino acid
  • Anticodon loop
  • Fluorescence
  • Mg
  • tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Physical and Theoretical Chemistry

Cite this

Contributions to selective binding of aromatic amino acid residues to tRNAPhe . / Bujalowski, Wlodzimierz; Porschke, D.

In: Biophysical Chemistry, Vol. 30, No. 2, 1988, p. 151-157.

Research output: Contribution to journalArticle

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