Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination

Vaniyambadi V. Sridhar, Avnish Kapoor, Kangling Zhang, Jianjun Zhu, Tao Zhou, Paul M. Hasegawa, Ray A. Bressan, Jian Kang Zhu

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Epigenetic regulation involves reversible changes in DNA methylation and/or histone modification patterns. Short interfering RNAs (siRNAs) can direct DNA methylation and heterochromatic histone modifications, causing sequence-specific transcriptional gene silencing. In animals and yeast, histone H2B is known to be monoubiquitinated, and this regulates the methylation of histone H3 (refs 10, 11). However, the relationship between histone ubiquitination and DNA methylation has not been investigated. Here we show that mutations in an Arabidopsis deubiquitination enzyme, SUP32/UBP26, decrease the dimethylation on lysine 9 of H3, suppress siRNA-directed methylation of DNA and release heterochromatic silencing of transgenes as well as transposons. We found that Arabidopsis histone H2B is monoubiquitinated at lysine 143 and that the levels of ubiquitinated H2B and trimethyl H3 at lysine 4 increase in sup32 mutant plants. SUP32/UBP26 can deubiquitinate H2B, and chromatin immunoprecipitation assays suggest an association between H2B ubiquitination and release of silencing. These data suggest that H2B deubiquitination by SUP32/UBP26 is required for heterochromatic histone H3 methylation and DNA methylation.

Original languageEnglish (US)
Pages (from-to)735-738
Number of pages4
JournalNature
Volume447
Issue number7145
DOIs
StatePublished - Jun 7 2007
Externally publishedYes

Fingerprint

DNA Methylation
Histones
Histone Code
Lysine
Ubiquitination
Arabidopsis
Methylation
Small Interfering RNA
Chromatin Immunoprecipitation
Gene Silencing
Transgenes
Epigenomics
Yeasts
Mutation
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Sridhar, V. V., Kapoor, A., Zhang, K., Zhu, J., Zhou, T., Hasegawa, P. M., ... Zhu, J. K. (2007). Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination. Nature, 447(7145), 735-738. https://doi.org/10.1038/nature05864

Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination. / Sridhar, Vaniyambadi V.; Kapoor, Avnish; Zhang, Kangling; Zhu, Jianjun; Zhou, Tao; Hasegawa, Paul M.; Bressan, Ray A.; Zhu, Jian Kang.

In: Nature, Vol. 447, No. 7145, 07.06.2007, p. 735-738.

Research output: Contribution to journalArticle

Sridhar, VV, Kapoor, A, Zhang, K, Zhu, J, Zhou, T, Hasegawa, PM, Bressan, RA & Zhu, JK 2007, 'Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination', Nature, vol. 447, no. 7145, pp. 735-738. https://doi.org/10.1038/nature05864
Sridhar, Vaniyambadi V. ; Kapoor, Avnish ; Zhang, Kangling ; Zhu, Jianjun ; Zhou, Tao ; Hasegawa, Paul M. ; Bressan, Ray A. ; Zhu, Jian Kang. / Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination. In: Nature. 2007 ; Vol. 447, No. 7145. pp. 735-738.
@article{24238776979a435fa95b7b06e1329b93,
title = "Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination",
abstract = "Epigenetic regulation involves reversible changes in DNA methylation and/or histone modification patterns. Short interfering RNAs (siRNAs) can direct DNA methylation and heterochromatic histone modifications, causing sequence-specific transcriptional gene silencing. In animals and yeast, histone H2B is known to be monoubiquitinated, and this regulates the methylation of histone H3 (refs 10, 11). However, the relationship between histone ubiquitination and DNA methylation has not been investigated. Here we show that mutations in an Arabidopsis deubiquitination enzyme, SUP32/UBP26, decrease the dimethylation on lysine 9 of H3, suppress siRNA-directed methylation of DNA and release heterochromatic silencing of transgenes as well as transposons. We found that Arabidopsis histone H2B is monoubiquitinated at lysine 143 and that the levels of ubiquitinated H2B and trimethyl H3 at lysine 4 increase in sup32 mutant plants. SUP32/UBP26 can deubiquitinate H2B, and chromatin immunoprecipitation assays suggest an association between H2B ubiquitination and release of silencing. These data suggest that H2B deubiquitination by SUP32/UBP26 is required for heterochromatic histone H3 methylation and DNA methylation.",
author = "Sridhar, {Vaniyambadi V.} and Avnish Kapoor and Kangling Zhang and Jianjun Zhu and Tao Zhou and Hasegawa, {Paul M.} and Bressan, {Ray A.} and Zhu, {Jian Kang}",
year = "2007",
month = "6",
day = "7",
doi = "10.1038/nature05864",
language = "English (US)",
volume = "447",
pages = "735--738",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7145",

}

TY - JOUR

T1 - Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination

AU - Sridhar, Vaniyambadi V.

AU - Kapoor, Avnish

AU - Zhang, Kangling

AU - Zhu, Jianjun

AU - Zhou, Tao

AU - Hasegawa, Paul M.

AU - Bressan, Ray A.

AU - Zhu, Jian Kang

PY - 2007/6/7

Y1 - 2007/6/7

N2 - Epigenetic regulation involves reversible changes in DNA methylation and/or histone modification patterns. Short interfering RNAs (siRNAs) can direct DNA methylation and heterochromatic histone modifications, causing sequence-specific transcriptional gene silencing. In animals and yeast, histone H2B is known to be monoubiquitinated, and this regulates the methylation of histone H3 (refs 10, 11). However, the relationship between histone ubiquitination and DNA methylation has not been investigated. Here we show that mutations in an Arabidopsis deubiquitination enzyme, SUP32/UBP26, decrease the dimethylation on lysine 9 of H3, suppress siRNA-directed methylation of DNA and release heterochromatic silencing of transgenes as well as transposons. We found that Arabidopsis histone H2B is monoubiquitinated at lysine 143 and that the levels of ubiquitinated H2B and trimethyl H3 at lysine 4 increase in sup32 mutant plants. SUP32/UBP26 can deubiquitinate H2B, and chromatin immunoprecipitation assays suggest an association between H2B ubiquitination and release of silencing. These data suggest that H2B deubiquitination by SUP32/UBP26 is required for heterochromatic histone H3 methylation and DNA methylation.

AB - Epigenetic regulation involves reversible changes in DNA methylation and/or histone modification patterns. Short interfering RNAs (siRNAs) can direct DNA methylation and heterochromatic histone modifications, causing sequence-specific transcriptional gene silencing. In animals and yeast, histone H2B is known to be monoubiquitinated, and this regulates the methylation of histone H3 (refs 10, 11). However, the relationship between histone ubiquitination and DNA methylation has not been investigated. Here we show that mutations in an Arabidopsis deubiquitination enzyme, SUP32/UBP26, decrease the dimethylation on lysine 9 of H3, suppress siRNA-directed methylation of DNA and release heterochromatic silencing of transgenes as well as transposons. We found that Arabidopsis histone H2B is monoubiquitinated at lysine 143 and that the levels of ubiquitinated H2B and trimethyl H3 at lysine 4 increase in sup32 mutant plants. SUP32/UBP26 can deubiquitinate H2B, and chromatin immunoprecipitation assays suggest an association between H2B ubiquitination and release of silencing. These data suggest that H2B deubiquitination by SUP32/UBP26 is required for heterochromatic histone H3 methylation and DNA methylation.

UR - http://www.scopus.com/inward/record.url?scp=34250025664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250025664&partnerID=8YFLogxK

U2 - 10.1038/nature05864

DO - 10.1038/nature05864

M3 - Article

VL - 447

SP - 735

EP - 738

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7145

ER -