Control of gonadotropin secretion by follicle-stimulating hormone-releasing factor, luteinizing hormone-releasing hormone, and leptin

S. M. McCann, S. Karanth, C. A. Mastronardi, W. Les Dees, G. Childs, B. Miller, S. Sower, W. H. Yu

    Research output: Contribution to journalArticle

    51 Citations (Scopus)

    Abstract

    Fractionation of hypothalamic extracts on a Sephadex G-25 column separates follicle-stimulating hormone-releasing factor (FSHRF) from luteinizing hormone-releasing hormone (LHRH). The FSH-releasing peak contained immunoreactive lamprey gonadotropin-releasing hormone (lGnRH) by radioimmunoassay, and its activity was inactivated by an antiserum specific to lGnRH. The identity of lGnRH-III with FSHRF is supported by studies with over 40 GnRH analogs that revealed that this is the sole analog with preferential FSH-releasing activity. Selective activity appears to require amino acids 5-8 of lGnRH-III. Chicken GnRH-II has slight selective FSH-releasing activity. Using a specific lGnRH-III antiserum, a population of lGnRH-III neurons was visualized in the dorsal and ventral preoptic area with axons projecting to the median eminence in areas shown previously to control FSH secretion based on lesion and stimulation studies. Some lGnRH-III neurons contained only this peptide, others also contained LHRH, and still others contained only LHRH. The differential pulsatile release of FSH and LH and their differential secretion at different times of the estrous cycle may be caused by differential secretion of FSHRF and LHRH. Both FSH and LHRH act by nitric oxide (NO) that generates cyclic guanosine monophosphate. lGnRH-III has very low affinity to the LHRH receptor. Biotinylated lGnRH-III (10-9 M) labels 80% of FSH gonadotropes and is not displaced by LHRH, providing evidence for the existence of an FSHRF receptor. Leptin has equal potency as LHRH to release gonadotropins by NO. lGnRH-III specifically releases FSH, not only in rats but also in cows.

    Original languageEnglish (US)
    Pages (from-to)476-485
    Number of pages10
    JournalArchives of Medical Research
    Volume32
    Issue number6
    DOIs
    StatePublished - 2001

    Fingerprint

    Lampreys
    Follicle Stimulating Hormone
    Leptin
    Gonadotropins
    Gonadotropin-Releasing Hormone
    Immune Sera
    Nitric Oxide
    LHRH Receptors
    Neurons
    Median Eminence
    Preoptic Area
    Estrous Cycle
    gonadotropin-releasing hormone-III
    Cyclic GMP
    Radioimmunoassay
    Axons
    Chickens

    Keywords

    • FHS
    • FSHRF
    • GnRH
    • Gonadotropin secretion
    • Leptin
    • LHRH

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Control of gonadotropin secretion by follicle-stimulating hormone-releasing factor, luteinizing hormone-releasing hormone, and leptin. / McCann, S. M.; Karanth, S.; Mastronardi, C. A.; Les Dees, W.; Childs, G.; Miller, B.; Sower, S.; Yu, W. H.

    In: Archives of Medical Research, Vol. 32, No. 6, 2001, p. 476-485.

    Research output: Contribution to journalArticle

    McCann, S. M. ; Karanth, S. ; Mastronardi, C. A. ; Les Dees, W. ; Childs, G. ; Miller, B. ; Sower, S. ; Yu, W. H. / Control of gonadotropin secretion by follicle-stimulating hormone-releasing factor, luteinizing hormone-releasing hormone, and leptin. In: Archives of Medical Research. 2001 ; Vol. 32, No. 6. pp. 476-485.
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    AU - McCann, S. M.

    AU - Karanth, S.

    AU - Mastronardi, C. A.

    AU - Les Dees, W.

    AU - Childs, G.

    AU - Miller, B.

    AU - Sower, S.

    AU - Yu, W. H.

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