@inproceedings{4d0e9a8b41c24b9e85f4854a4492a3eb,
title = "Controlling microarray DNA hybridization efficiency by probe-surface distance and external surface electrostatics",
abstract = "DNA microarrays are analytical devices designed to determine the composition of multicomponent solutions of nucleic acids, DNA or RNA. These devices are promising technology for diverse applications, including sensing, diagnostics, and drug/gene delivery. Here, we modify a hybridization adsorption isotherm to study the effects of probe-surface distance and the external electrostatic fields, on the oligonucleotide hybridization in microarray and how these effects are varies depending on surface probe density and target concentration. This study helps in our understanding on-surface hybridization mechanisms, and from it we can observe a significant effect of the probe-surface distance, and the external electrostatic fields, on the hybridization yield. In addition we present a simple new criteria to control the oligonucleotide hybridization efficiency by providing a chart illustrating the effects of all factors on the DNA-hybridization efficiency.",
keywords = "DNA Hybridization, Linker, Microarrays, probes, surface Electrostatic",
author = "K. Qamhieh and Pettitt, {B. Montgomery}",
note = "Publisher Copyright: {\textcopyright} 2015 AIP Publishing LLC.; 4th International Congress in Advances in Applied Physics and Materials Science, APMAS 2014 ; Conference date: 24-04-2014 Through 27-04-2014",
year = "2015",
month = mar,
day = "30",
doi = "10.1063/1.4914281",
language = "English (US)",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Bahsi, {Zehra Banu} and Okoz, {Mehmet Emre} and Oral, {Ahmet Yavuz} and Mehmet Ozer and Mehmet Sezer",
booktitle = "4th International Congress in Advances in Applied Physics and Materials Science, APMAS 2014",
}