Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies

Charles D. Murin, Pavlo Gilchuk, Philipp A. Ilinykh, Kai Huang, Natalia Kuzmina, Xiaoli Shen, Jessica F. Bruhn, Aubrey L. Bryan, Edgar Davidson, Benjamin J. Doranz, Lauren E. Williamson, Jeffrey Copps, Tanwee Alkutkar, Andrew I. Flyak, Alexander Bukreyev, James E. Crowe, Andrew B. Ward

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    Antibodies that target the glycan cap epitope on the ebolavirus glycoprotein (GP) are common in the adaptive response of survivors. A subset is known to be broadly neutralizing, but the details of their epitopes and basis for neutralization are not well understood. Here, we present cryoelectron microscopy (cryo-EM) structures of diverse glycan cap antibodies that variably synergize with GP base-binding antibodies. These structures describe a conserved site of vulnerability that anchors the mucin-like domains (MLDs) to the glycan cap, which we call the MLD anchor and cradle. Antibodies that bind to the MLD cradle share common features, including use of IGHV1-69 and IGHJ6 germline genes, which exploit hydrophobic residues and form β-hairpin structures to mimic the MLD anchor, disrupt MLD attachment, destabilize GP quaternary structure, and block cleavage events required for receptor binding. Our results provide a molecular basis for ebolavirus neutralization by broadly reactive glycan cap antibodies.

    Original languageEnglish (US)
    Article number108984
    JournalCell Reports
    Volume35
    Issue number2
    DOIs
    StatePublished - Apr 13 2021

    Keywords

    • Ebola virus
    • antibody
    • antibody therapeutics
    • broadly neutralizing
    • ebolaviruses
    • filoviruses
    • glycan cap
    • mAb

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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