Conversion of PtdIns(4, 5)P2 into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology

Kirsten Niebuhr, Sylvie Giuriato, Thierry Pedron, Dana J. Philpott, Frédérique Gaits, Julia Sable, Michael Sheetz, Claude Parsot, Philippe J. Sansonetti, Bernard Payrastre

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

Phosphoinositides play a central role in the control of several cellular events including actin cytoskeleton organization. Here we show that, upon infection of epithelial cells with the Gram-negative pathogen Shigella flexneri, the virulence factor IpgD is translocated directly into eukaryotic cells and acts as a potent inositol 4-phosphatase that specifically dephosphorylates phosphatidylinositol 4, 5-bisphosphate [PtdIns- (4, 5)P2] into phosphatidylinositol 5-monophosphate [PtdIns(5)P] that then accumulates. Transfection experiments indicate that the transformation of PtdIns(4, 5)P2 into PtdIns(5)P by IpgD is responsible for dramatic morphological changes of the host cell, leading to a decrease in membrane tether force associated with membrane blebbing and actin filament remodelling. These data provide the molecular basis for a new mechanism employed by a pathogenic bacterium to promote membrane ruffling at the entry site.

Original languageEnglish (US)
Pages (from-to)5069-5078
Number of pages10
JournalEMBO Journal
Volume21
Issue number19
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Phosphatidylinositol 4,5-Diphosphate
Phosphatidylinositols
Membranes
Actin Cytoskeleton
Actins
Shigella flexneri
Eukaryotic Cells
Virulence Factors
Pathogens
Inositol
Blister
Phosphoric Monoester Hydrolases
Transfection
Bacteria
Epithelial Cells
Infection
Experiments

Keywords

  • Bacterial entry
  • IpgD
  • Phosphoinositides
  • S.flexneri

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Niebuhr, K., Giuriato, S., Pedron, T., Philpott, D. J., Gaits, F., Sable, J., ... Payrastre, B. (2002). Conversion of PtdIns(4, 5)P2 into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology. EMBO Journal, 21(19), 5069-5078. https://doi.org/10.1093/emboj/cdf522

Conversion of PtdIns(4, 5)P2 into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology. / Niebuhr, Kirsten; Giuriato, Sylvie; Pedron, Thierry; Philpott, Dana J.; Gaits, Frédérique; Sable, Julia; Sheetz, Michael; Parsot, Claude; Sansonetti, Philippe J.; Payrastre, Bernard.

In: EMBO Journal, Vol. 21, No. 19, 01.10.2002, p. 5069-5078.

Research output: Contribution to journalArticle

Niebuhr, K, Giuriato, S, Pedron, T, Philpott, DJ, Gaits, F, Sable, J, Sheetz, M, Parsot, C, Sansonetti, PJ & Payrastre, B 2002, 'Conversion of PtdIns(4, 5)P2 into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology', EMBO Journal, vol. 21, no. 19, pp. 5069-5078. https://doi.org/10.1093/emboj/cdf522
Niebuhr, Kirsten ; Giuriato, Sylvie ; Pedron, Thierry ; Philpott, Dana J. ; Gaits, Frédérique ; Sable, Julia ; Sheetz, Michael ; Parsot, Claude ; Sansonetti, Philippe J. ; Payrastre, Bernard. / Conversion of PtdIns(4, 5)P2 into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology. In: EMBO Journal. 2002 ; Vol. 21, No. 19. pp. 5069-5078.
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