Cooperative binding of polyamines induces the Escherichia coli single-strand binding protein-DNA binding mode transitions

Tai Fen Wei, Wlodzimierz Bujalowski, Timothy M. Lohman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer and in the type and degree of cooperative complexes that are formed with ss DNA. Although it is not yet known whether only one or all of these modes function in vivo, based on the dramatically different properties of the SSB tetramer in these different ss DNA binding modes, it has been suggested that the different modes may function selectively in replication, recombination, and/or repair. The transitions between these different modes are very sensitive to solution conditions, including salt (concentration, as well as cation and anion type), pH, and temperature. We have examined the effects of multivalent cations, principally the polyamine spermine, on the SSB-ss poly(dT) binding mode transitions and find that the transition from the (SSB)35 to the (SSB)56 binding mode can be induced by micromolar concentrations of polyamines as well as the inorganic cation Co(NH3)6 3+. Furthermore, these multivalent cations, as well as Mg2+, induce the binding mode transition by binding cooperatively to the SSB-poly(dT) complexes. These observations are interesting in light of the fact that polyamines, such as spermidine, are part of the ionic environment in E. coli and hence these cations are likely to affect the distribution of SSB-ss DNA binding modes in vivo. Furthermore, the ability of the SSB protein to enhance the rate of renaturation of complementary single-stranded DNA >5000-fold is directly dependent upon the presence of polyamines [Christiansen, C., & Baldwin, R. L. (1977) J. Mol. Biol. 115, 441].

Original languageEnglish (US)
Pages (from-to)6166-6174
Number of pages9
JournalBiochemistry
Volume31
Issue number26
StatePublished - 1992
Externally publishedYes

Fingerprint

DNA-Binding Proteins
Polyamines
Escherichia coli
Cations
Poly T
Recombinational DNA Repair
DNA
Carrier Proteins
Repair
Spermidine
Spermine
Single-Stranded DNA
DNA Replication
DNA Repair
Nucleic Acids
Anions
Proteins
Nucleotides
Complementary DNA
Salts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cooperative binding of polyamines induces the Escherichia coli single-strand binding protein-DNA binding mode transitions. / Wei, Tai Fen; Bujalowski, Wlodzimierz; Lohman, Timothy M.

In: Biochemistry, Vol. 31, No. 26, 1992, p. 6166-6174.

Research output: Contribution to journalArticle

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