Coptidis Rhizoma extract inhibits replication of respiratory syncytial virus in vitro and in vivo by inducing antiviral state

Byeong Hoon Lee, Kiramage Chathuranga, Md Bashir Uddin, Prasanna Weeratunga, Myun Soo Kim, Won Kyung Cho, Hong Ik Kim, Jin Yeul Ma, Jong Soo Lee

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Coptidis Rhizoma is derived from the dried rhizome of Ranunculaceous plants and is a commonly used traditional Chinese medicine. Although Coptidis Rhizoma is commonly used for its many therapeutic effects, antiviral activity against respiratory syncytial virus (RSV) has not been reported in detail. In this study, we evaluated the antiviral activities of Coptidis Rhizoma extract (CRE) against RSV in human respiratory tract cell line (HEp2) and BALB/c mice. An effective dose of CRE significantly reduces the replication of RSV in HEp2 cells and reduces the RSV-induced cell death. This antiviral activity against RSV was through the induction of type I interferon-related signaling and the antiviral state in HEp2 cells. More importantly, oral administration of CRE exhibited prophylactic effects in BALB/c mice against RSV. In HPLC analysis, we found the presence of several compounds in the aqueous fraction and among them; we confirmed that palmatine was related to the antiviral properties and immunemodulation effect. Taken together, an extract of Coptidis Rhizoma and its components play roles as immunomodulators and could be a potential source as promising natural antivirals that can confer protection to RSV. These outcomes should encourage further allied studies in other natural products.

Original languageEnglish (US)
Pages (from-to)488-498
Number of pages11
JournalJournal of Microbiology
Volume55
Issue number6
DOIs
StatePublished - Jun 1 2017
Externally publishedYes

Keywords

  • antiviral effect
  • coptidis rhizoma (CR)
  • palmatine
  • respiratory syncytial virus (RSV)

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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