Coronavirus transcription early in infection

Sungwhan An, Akihiko Maeda, Shinji Makino

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We studied the accumulation kinetics of murine coronavirus mouse hepatitis virus (MHV) RNAs early in infection by using cloned MHV defective interfering (DI) RNA that contained an intergenic sequence from which subgenomic DI RNA is synthesized in MHV-infected cells. Genomic DI RNA and subgenomic DI RNA accumulated at a constant ratio from 3 to 11 h postinfection (p.i.) in the cells infected with MHV-containing DI particles. Earlier, at 1 h p.i., this ratio was not constant; only genomic DI RNA accumulated, indicating that MHV RNA replication, but not MHV RNA transcription, was active during the first hour of MHV infection. Negative- strand genomic DI RNA and negative-strand subgenomic DI RNA were first detectable at 1 and 3 h p.i., respectively, and the amounts of both RNAs increased gradually until 6 h p.i. These data showed that at 2 h p.i., subgenomic DI RNA was undergoing synthesis in the cells in which negative- strand subgenomic DI RNA was undetectable. These data, therefore, signify that negative-strand genomic DI RNA, but not negative-strand subgenomic DI RNA, was an active template for subgenomic DI RNA synthesis early in infection.

Original languageEnglish (US)
Pages (from-to)8517-8524
Number of pages8
JournalJournal of Virology
Volume72
Issue number11
StatePublished - Nov 1998
Externally publishedYes

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Coronavirus
Coronavirinae
Murine hepatitis virus
transcription (genetics)
RNA
Infection
infection
genomics
Murine coronavirus
Defective Viruses
synthesis
Intergenic DNA
cells
intergenic DNA
Virus Diseases
Virus Replication

ASJC Scopus subject areas

  • Immunology

Cite this

Coronavirus transcription early in infection. / An, Sungwhan; Maeda, Akihiko; Makino, Shinji.

In: Journal of Virology, Vol. 72, No. 11, 11.1998, p. 8517-8524.

Research output: Contribution to journalArticle

An, S, Maeda, A & Makino, S 1998, 'Coronavirus transcription early in infection', Journal of Virology, vol. 72, no. 11, pp. 8517-8524.
An, Sungwhan ; Maeda, Akihiko ; Makino, Shinji. / Coronavirus transcription early in infection. In: Journal of Virology. 1998 ; Vol. 72, No. 11. pp. 8517-8524.
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