Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors

M. Montanari, C. Gemelli, E. Tenedini, T. Zanocco-Marani, T. Vignudelli, M. Siena, R. Zini, S. Salati, G. Chiossi, E. Tagliafico, R. Manfredini, A. Grande, Sergio Ferrari

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

In spite of their apparently restricted differentiation potentiality, hematopoietic precursors are plastic cells able to trans-differentiate from a maturation lineage to another. To better characterize this differentiation plasticity, we purified CD14- and CD14+ myeloid precursors generated by 'in vitro' culture of human CD34+ hematopoietic progenitors. Morphological analysis of the investigated cell populations indicated that, as expected, they consisted of granulocyte and monocyte precursors, respectively. Treatment with differentiation inducers revealed that CD14- cells were bipotent granulo-monocyte precursors, while CD14+ cells appeared univocally committed to a terminal macrophage maturation. Flow cytometry analysis demonstrated that the conversion of granulocyte precursors to the mono-macrophage maturation lineage occurs through a differentiation transition in which the granulocyte-related myeloperoxidase enzyme and the monocyte-specific CD14 antigen are co-expressed. Expression profiling evidenced that the observed trans-differentiation process was accompanied by a remarkable upregulation of the monocyte-related MafB transcription factor.

Original languageEnglish (US)
Pages (from-to)1588-1600
Number of pages13
JournalCell Death and Differentiation
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2005

Keywords

  • Lineage switching
  • Normal myeloid precursors
  • Trans-differentiation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Montanari, M., Gemelli, C., Tenedini, E., Zanocco-Marani, T., Vignudelli, T., Siena, M., Zini, R., Salati, S., Chiossi, G., Tagliafico, E., Manfredini, R., Grande, A., & Ferrari, S. (2005). Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors. Cell Death and Differentiation, 12(12), 1588-1600. https://doi.org/10.1038/sj.cdd.4401679