Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors

M. Montanari, C. Gemelli, E. Tenedini, T. Zanocco-Marani, T. Vignudelli, M. Siena, R. Zini, S. Salati, Giuseppe Chiossi, E. Tagliafico, R. Manfredini, A. Grande, Sergio Ferrari

Research output: Contribution to journalArticle

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Abstract

In spite of their apparently restricted differentiation potentiality, hematopoietic precursors are plastic cells able to trans-differentiate from a maturation lineage to another. To better characterize this differentiation plasticity, we purified CD14- and CD14+ myeloid precursors generated by 'in vitro' culture of human CD34+ hematopoietic progenitors. Morphological analysis of the investigated cell populations indicated that, as expected, they consisted of granulocyte and monocyte precursors, respectively. Treatment with differentiation inducers revealed that CD14- cells were bipotent granulo-monocyte precursors, while CD14+ cells appeared univocally committed to a terminal macrophage maturation. Flow cytometry analysis demonstrated that the conversion of granulocyte precursors to the mono-macrophage maturation lineage occurs through a differentiation transition in which the granulocyte-related myeloperoxidase enzyme and the monocyte-specific CD14 antigen are co-expressed. Expression profiling evidenced that the observed trans-differentiation process was accompanied by a remarkable upregulation of the monocyte-related MafB transcription factor.

Original languageEnglish (US)
Pages (from-to)1588-1600
Number of pages13
JournalCell Death and Differentiation
Volume12
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Monocytes
Granulocytes
Messenger RNA
MafB Transcription Factor
Macrophages
CD14 Antigens
Peroxidase
Plastics
Flow Cytometry
Up-Regulation
Enzymes
Population

Keywords

  • Lineage switching
  • Normal myeloid precursors
  • Trans-differentiation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors. / Montanari, M.; Gemelli, C.; Tenedini, E.; Zanocco-Marani, T.; Vignudelli, T.; Siena, M.; Zini, R.; Salati, S.; Chiossi, Giuseppe; Tagliafico, E.; Manfredini, R.; Grande, A.; Ferrari, Sergio.

In: Cell Death and Differentiation, Vol. 12, No. 12, 12.2005, p. 1588-1600.

Research output: Contribution to journalArticle

Montanari, M, Gemelli, C, Tenedini, E, Zanocco-Marani, T, Vignudelli, T, Siena, M, Zini, R, Salati, S, Chiossi, G, Tagliafico, E, Manfredini, R, Grande, A & Ferrari, S 2005, 'Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors', Cell Death and Differentiation, vol. 12, no. 12, pp. 1588-1600. https://doi.org/10.1038/sj.cdd.4401679
Montanari, M. ; Gemelli, C. ; Tenedini, E. ; Zanocco-Marani, T. ; Vignudelli, T. ; Siena, M. ; Zini, R. ; Salati, S. ; Chiossi, Giuseppe ; Tagliafico, E. ; Manfredini, R. ; Grande, A. ; Ferrari, Sergio. / Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors. In: Cell Death and Differentiation. 2005 ; Vol. 12, No. 12. pp. 1588-1600.
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