TY - JOUR
T1 - Correlation of Biochemical and Hematological Changes with Graft Failure Following Pig Heart and Kidney Transplantation in Baboons
AU - Knosalla, Christoph
AU - Gollackner, Bernd
AU - Bühler, Leo
AU - Mueller, Nicolas J.
AU - Houser, Stuart
AU - Mauiyyedi, Shamila
AU - Sachs, David H.
AU - Robson, Simon C.
AU - Fishman, Jay
AU - Schuurman, Henk Jan
AU - Awwad, Michel
AU - Cooper, David K.C.
PY - 2003/12
Y1 - 2003/12
N2 - We have explored biochemical and hematologic parameters that might indicate acute humoral xenograft rejection (AHXR) following pig organ transplantation in baboons. Baboons (n = 15) received an immunosuppressive regimen, and underwent a miniature swine or hDAF kidney (Group 1, n = 6) or heart (Group 2, n = 7) transplantation. Control baboons (Group 3, n = 2) received the immunosuppressive regimen without organ transplantation. Blood chemistry and hematologic parameters were measured daily. Baboon and porcine cytomegalovirus were monitored. In Groups 1 and 2, organ grafts survived for up to 29 days. A plasma fibrinogen of <80 mg/dL on 2 consecutive days, and a serum lactate dehydrogenase of >600 U/L and aspartate transaminase of >300 U/L, were associated with the development of AHXR in both heart and kidney grafts. In Group 1, a decrease in platelet count of >150 000/μL within 3 days, or a count of <50 000/μL, were associated with AHXR. In Group 2, a creatine phosphokinase of >500 U/L was associated with graft failure. In Group 3, no abnormalities were observed. The possibility that porcine CMV may play a role in graft injury could not be excluded. Noninvasive parameters were identified that have predictive potential for AHXR. Monitoring of these might enable therapeutic intervention to reverse rejection.
AB - We have explored biochemical and hematologic parameters that might indicate acute humoral xenograft rejection (AHXR) following pig organ transplantation in baboons. Baboons (n = 15) received an immunosuppressive regimen, and underwent a miniature swine or hDAF kidney (Group 1, n = 6) or heart (Group 2, n = 7) transplantation. Control baboons (Group 3, n = 2) received the immunosuppressive regimen without organ transplantation. Blood chemistry and hematologic parameters were measured daily. Baboon and porcine cytomegalovirus were monitored. In Groups 1 and 2, organ grafts survived for up to 29 days. A plasma fibrinogen of <80 mg/dL on 2 consecutive days, and a serum lactate dehydrogenase of >600 U/L and aspartate transaminase of >300 U/L, were associated with the development of AHXR in both heart and kidney grafts. In Group 1, a decrease in platelet count of >150 000/μL within 3 days, or a count of <50 000/μL, were associated with AHXR. In Group 2, a creatine phosphokinase of >500 U/L was associated with graft failure. In Group 3, no abnormalities were observed. The possibility that porcine CMV may play a role in graft injury could not be excluded. Noninvasive parameters were identified that have predictive potential for AHXR. Monitoring of these might enable therapeutic intervention to reverse rejection.
KW - Acute humoral xenograft rejection
KW - Baboon
KW - Consumptive coagulopathy
KW - Endothelial cell activation
KW - Heart transplantation
KW - Kidney transplantation
KW - Pig
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U2 - 10.1046/j.1600-6135.2003.00258.x
DO - 10.1046/j.1600-6135.2003.00258.x
M3 - Article
C2 - 14629281
AN - SCOPUS:0346121379
SN - 1600-6135
VL - 3
SP - 1510
EP - 1519
JO - American Journal of Transplantation
JF - American Journal of Transplantation
IS - 12
ER -